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Effects of Deltamethrin in muscular acetylcholinesterase of Brycon amazonicus

Grant number: 12/07150-5
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): January 01, 2013
Effective date (End): December 31, 2013
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
Principal Investigator:Gilberto Moraes
Grantee:Camila Aparecida Pigão Soares
Home Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil


Over the last years, insecticides less toxic and less persisting into mammalian organisms, such as pyrethroids, have been used. Some studies have indicated that these insecticides are highly active to non target aquatic organisms. Deltamethrin is a type II pyrethroid, is very toxic to crustaceans and fish even at low concentrations. It inhibits nicotinic acetylcholine receptors, channel voltage-dependent Ca+2 and channel voltage-dependent Na+2 of neuronal membrane. Fish is very sensible to many xenobiotics allowing their use as way of evaluating the environmental pollution. The organisms able to highlight biological responses to xenobiotics are said bioindicators. Their responses are the set of alterations in the homeostasis of the organism. Acetylcholinesterase (AChE) is a bioindicator able to hydrolyze acetylcholine into choline and acetate. In this project, that enzyme will be used to evaluate the effects of deltamethrin on the muscle of the teleost matrinxã Brycon cephalus. The fish will be exposed for 4, 24 e 48 hours to 0.26µg/L of the deltamethrin (10% of the LC;50) in a system of tanks "in door". After this period, the fish will be anesthetized and killed to excision of white muscle and assay of AChE activity.