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IDENTIFICATION, MOLECULAR CHARACTERIZATION, MAPPING AND COLOCALIZATION OF THE RECEPTOR 1 MELANIN-CONCENTRATING HORMONE IN MAMMARY GLAND OF LACTATING AND NON-LACTATING RATS.

Grant number: 12/04554-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2012
Effective date (End): February 29, 2016
Field of knowledge:Biological Sciences - Morphology - Anatomy
Principal researcher:Jackson Cioni Bittencourt
Grantee:Daniella Sabino Battagello
Home Institution: Instituto de Psicologia (IP). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Introduction: The melanin-concentrating hormone (MCH) has the highest expression of its messenger RNA (mRNA) for the last 10 days of lactation (specifically in the 19 days) in unusual areas in the central nervous system (CNS) of rodent, such as the medial preoptic area (MPOA). After this period no longer find neither the mRNA nor the protein in the MPOA. Which leads us to believe that the MCH has a role in the final period of lactation. It has also been described that there are fluctuations in serum of the MCH. It has been reported the presence of MCH in various organs such as lung, thyroid, spleen, gastrointestinal tract and especially of the CNS that is responsible for expression of 98% of the peptide. Two receptors have been described MCH, the MCHR1 in CNS in rodents and MCHR2 CNS rodent and human. However, there is no description in the presence of MCHR1 breast tissue, although evidence support a possible relationship between the MCH and the period of lactation. Objectives: In this way we aim to: 1) to establish the serum levels of MCH during lactation and beyond, 2) investigate the presence of MCHR1 receptors in breast tissue, 3) identify the cell type which expresses mRNA and protein, and 4) sequence found on the recipient rat breast to check the degree of homology with the receptor found in the central nervous system. Material and Methods: For this, we will use methods such as: 1) radioimmunoassay for determining serum levels of MCH before, during and after lactation, 2) immunohistochemical staining for the receptor MCHR1 in mammary tissue of rats at different stages of the cycle and estrus periods established lactation (5, 12, 15 and 20 days) for checking the type of cell which expresses the mRNA of MCHR1, 3) the use of in situ hybridization method for studying the expression of mRNA in different MCHR1 periods of lactation and beyond, and 4) using the technique of polymerase chain reaction (PCR) for molecular characterization of MCHR1. Preliminary results, using the technique of immunohistochemistry might have evidence of the presence of MCHR1 in breast tissue. Conclusion: Thus, we can infer from these preliminary results that MCHR1 is present in breast tissue, but we still have to characterize the cell type, the degree of homology, confirming the periods of expression of mRNA and protein and correlate with serum MCH before, during and after the event of lactation.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BATTAGELLO, DANIELLA S.; LORENZON, ALINE R.; DINIZ, GIOVANNE B.; MOTTA-TEIXEIRA, LIVIA C.; KLEIN, MARIANNE O.; FERREIRA, JOZELIA G. P.; ARIAS, CARLOS M.; ADAMANTIDIS, ANTOINE; SITA, V, LUCIANE; CIPOLLA-NETO, JOSE; BEVILACQUA, ESTELA M. A. F.; SAWCHENKO, PAUL E.; BITTENCOURT, JACKSON C. The Rat Mammary Gland as a Novel Site of Expression of Melanin-Concentrating Hormone Receptor 1 mRNA and Its Protein Immunoreactivity. FRONTIERS IN ENDOCRINOLOGY, v. 11, JUL 31 2020. Web of Science Citations: 0.
MOTTA-TEIXEIRA, LIVIA CLEMENTE; MACHADO-NILS, ALINE VILAR; BATTAGELLO, DANIELLA SABINO; DINIZ, GIOVANNE BARONI; ANDRADE-SILVA, JESSICA; SILVA JR, SINESIO; MATOS, RAPHAEL AFONSO; DO AMARAL, FERNANDA GASPAR; XAVIER, GILBERTO FERNANDO; BITTENCOURT, JACKSON CIONI; REITER, RUSSEL J.; LUCASSEN, PAUL JOHN; KOROSI, ANIKO; CIPOLLA-NETO, JOSE. The absence of maternal pineal melatonin rhythm during pregnancy and lactation impairs offspring physical growth, neurodevelopment, and behavior. Hormones and Behavior, v. 105, p. 146-156, SEP 2018. Web of Science Citations: 8.

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