Analysis of the expression of genes involved in epithelium mesenchyme transition in renal clear cell carcinoma

• Grant number: 12/08853-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: September 01, 2012
• End date: March 31, 2015
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Paula Rahal
• Grantee: Andre Luis Giacometti Conceicao
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

The renal tumor is the eighth most common cancer and highly lethal. It is a histologically heterogeneous disease, with the clear cell carcinoma (ccRCC), the most common histological subtype, comprising 70% to 80% of renal tumors. Although nephrectomy is a well established treatment, many patients develop metastasis. The ccRCC in the early stages, may change biological losing epithelial differentiation, a process called Epithelial Mesenchymal Transition (EMT). EMT is a change in molecular and cell coordinate, it is defined as a decrease in cell-cell adhesion, the areas of occlusion, the apical-basolateral polarity and mobility of the acquisition, aiding in the process of metastasis. This project aims to analyze the expression by RT-PCR (qRT-PCR) and immunohistochemical analysis of genes related to epithelial mesenchymal transition, and to evaluate the profile of genes methylation by MSP down-expression in renal cell carcinoma clear and renal carcinoma cell line treated and not treated with demethylating agent. Thus, the molecular and genetic characterizations allow a specific correlation between gene expression the methylation profile and the process of epithelial mesenchymal transition, in addition to the identification of potential markers that may contribute to the development of new therapeutic strategies. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CONCEICAO, ANDRE LUIS GIACOMETTI; DA SILVA, CAMILA TAINAH; BADIAL, RODOLFO MIGLIOLI; VALSECHI, MARINA CURADO; STUQUI, BRUNA; GONCALVES, JESSICA DOMINGUES; JASIULIONIS, MIRIAM GALVONAS; CALMON, MARILIA DE FREITAS; RAHAL, PAULA. Downregulation of OCLN and GAS1 in clear cell renal cell carcinoma. ONCOLOGY REPORTS, v. 37, n. 3, p. 1487-1496, MAR 2017. (12/08853-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CONCEICAO, Andre Luis Giacometti. Epithelial to mesenchymal transition related genes are differentially expressed in clear cell renal cell carcinoma. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto São José do Rio Preto.