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Study of the interaction between albumin and binuclear ruthenium carboxylates with potential biological activity through luminescence experiments

Grant number: 12/11682-2
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2012
Effective date (End): July 31, 2014
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Sofia Nikolaou
Grantee:Caroline Oliveira Sarmento
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

This project aims to bring a contribution investigating the interaction between the complexes [Ru2O (carb) 2 (py) 6] (PF6) 2 (carb = NSAIDs ibuprofen and ketoprofen) _ candidates _ metallo-drugs and biomolecules, specifically in the case of this application, bovine serum albumin (BSA) and human albumin (HSA).This project presents itself as a continuation of a previous project of Undergraduate Research was also funded by FAPESP in the form of grant (process number 2010/02717-1, Fellow Gabriela C. Seuanes). In the first stage of research, the binuclear ruthenium carboxylates of general formula [Ru2O (carb) 2 (py) 6] (PF6) 2 (carb = NSAIDs ibuprofen and ketoprofen), were synthesized and characterized by mass spectrometry techniques , NMR spectroscopy, UV-visible and infra-red, cyclic voltammetry and elemental analysis.At this time, aimed at finalizing the first stage of research and possibly the production of the first scientific study of the group addressing this class of compounds, we intend to investigate other variables of interest in terms of production of metallo-drug candidates, namely, its hospitalization with albumin. The study will focus on the spectroscopy of luminescence, by investigating the possible suppression of the luminescence of the tryptophan residues by the complex in question. This removal must be assessed by the curves and Stern-Volmer constants apparent adduct formation - protein complex also be determined.