Cell viability and bioactivity of materials based on Portland cement in primary culture cells of the follicle and dental human pulp, fibroblasts and osteoblasts

• Grant number: 12/08961-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2012
• End date: March 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Mario Tanomaru Filho
• Grantee: Leticia Boldrin Mestieri
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Studies on primary cell cultures represent important tool for the study of the toxic effects of severous dental materials in humans. The aim of this study is to isolate human cells of the follicle and the dental pulp of third molars subjected to extraction, to characterize these cells and keep them cryopreserved for testing bioactivity and cytotoxicity of new cements. For isolation of cells it will be tested two methods: explant + enzymatic treatment with trypsin and collagenase. Once the culture system of isolated cells is obtained, the same will be characterized by the use of specific markers for osteogenesis: bone morphogenic protein 7 (BMP-7) and dentin sialoprotein (DSP); and the formation of nodules of mineralization will be evaluated by the assays Alizarin Red and alkaline phosphatase. After maintaining the established cell culture, cytotoxicity assays will be performed (MTT and Trypan Blue) with the strains isolated by comparing them with two immortalized cell lines: fibroblasts (MPDL) and osteoblastic cells (SAOS-2), using the following cements experiments: 1) Portland cement (PC) 2) White MTA (Angelus), 3) + 30% CP ONB microparticulate; 4) CP + 30% nanoparticulate ONB

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LETICIA BOLDRIN MESTIERI; ANA LÍVIA GOMES-CORNÉLIO; ELISANDRA MÁRCIA RODRIGUES; GISELE FARIA; JULIANE MARIA GUERREIRO-TANOMARU; MÁRIO TANOMARU-FILHO. Cytotoxicity and Bioactivity of Calcium Silicate Cements Combined with Niobium Oxide in Different Cell Lines. Brazilian Dental Journal, v. 28, n. 1, p. 65-71, 2017-02-00. (12/13268-9, 12/08961-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MESTIERI, Leticia Boldrin. Estudo das células primárias dentais, viabilidade celular e bioatividade de materiais á base de silicato de cálcio. 2014. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.