Supplementation of feedlot cattle with selenium, vitamin E and canola oil on feed efficiency, meat quality and gene expression

• Grant number: 12/13666-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2012
• End date: July 31, 2014
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Principal Investigator: Arlindo Saran Netto
• Grantee: Gisele Fernanda Greghi
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

The present study aims to evaluate the effect of selenium, vitamin E and canola oil on feed efficiency, meat quality, activity of glutathione peroxidase and glutathione peroxidase gene expression of 1 and 4, reductase, synthase, nuclear factor erythroid Nrf2; delta-9 desaturase, SREBP-1c and HMG-CoA reductase. Forty-eight Nellore allocated to calan gate in the Department of Animal Science FZEA Pirassununga-USP, SP will be divided into four groups: Control (C) basal diet; Control + Antioxidants (C + A): addition of 2 , Se and 5 mg of vitamin E 500 IU / kg DM, Control + oil (C + O): addition of 3% canola oil / kg DM, oil and antioxidants (O + a): addition of 3% canola oil, 2.5 mg Se and 500 IU vitamin E / kg DM. Will be monitored daily dry matter intake and at weeks 0, 4, 8 and 12 will be weighed and have blood drawn to assess glucose, total cholesterol, triglycerides, selenium, vitamin E. At the end of the experiment, will be slaughtered, weighed, measured color, ribeye area, fat thickness and will be collected samples of liver, muscle (L. dorsi) and subcutaneous adipose tissue for analysis of Se, vitamin E, TBARS, profile fatty acids, cholesterol, softness, activity and gene expression of glutathione, the gene expression of Nrf2, delta-9-desaturase, SREBP-1c, and HMG-CoA reductase. The design is of a completely randomized design and the data will be analyzed by SAS PROC 5% significance level.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LATORRE, ANDREIA O.; GREGHI, GISELE F.; NETTO, ARLINDO SARAN; FUKUMASU, HEIDGE; BALIEIRO, JULIO C.; CORREA, LISIA B.; ZANETTI, MARCUS A.. Selenium and vitamin E enriched diet increases NK cell cytotoxicity in cattle. Pesquisa Veterinária Brasileira, v. 34, n. 11, p. 1141-1145, NOV 2014. (11/20987-9, 12/13666-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GREGHI, Gisele Fernanda. Evaluation of gene expression profile in cattle supplemented with selenium, vitamin E and canola oil: the interaction diet-gene-meat quality. 2014. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Zootecnica e Engenharia de Alimentos (FZE/BT) Pirassununga.