Metabolomics studies for the determination of cytotoxic activity of Chrysobalanaceae plant species From the Brazilian Cerrado

• Grant number: 12/20672-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: March 01, 2013
• End date: February 28, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Ian Castro-Gamboa
• Grantee: Fausto Carnevale Neto
• Supervisor: Daniel Raftery
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: University of Washington, United States
• Associated to the scholarship: 10/07564-9 - Study of Chrysobalanaceae species with cytotoxic activities: Metabolomic analysis for the understanding of sinergistic associations and their micromolecular complexity, BP.DR

Abstract

The search for bioactive substances in natural products has stimulated integrated approaches capable of providing information about the processes and interactions involved in the pharmacological responses of bioactive matrices. Among these approaches, highlights the metabolomics which, from the qualitative and quantitative analysis of the molecular composition of an organism, allows the explanation of biological processes through evaluation of metabolic responses, as well as an integrated view of the biochemistry of a complex mixture and its biological effects on a specific organism. This project aims to develop methods to assess the cytotoxic potential and the possible mechanisms involved in the bioactivity by integrated analysis of the metabolite profile of different tumor cell lines, using NMR and MS, against plant species from Chrysobalanaceae family. Different tumor cell lines will be treated with different concentrations of plant extracts and then analyzed by NMR and MS to determine the extra and intracellular metabolite profile. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GU, HAIWEI; CARROLL, PATRICK A.; DU, JIANHAI; ZHU, JIANGJIANG; NETO, FAUSTO CARNEVALE; EISENMAN, ROBERT N.; RAFTERY, DANIEL. Quantitative Method to Investigate the Balance between Metabolism and Proteome Biomass: Starting from Glycine. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, v. 55, n. 50, p. 15646-15650, DEC 2016. (12/20672-0)