Effects of Deltamethrin in muscular acetylcholinesterase of Brycon amazonicus

• Grant number: 12/07150-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2013
• End date: December 31, 2013
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Gilberto Moraes
• Grantee: Camila Aparecida Pigão Soares
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

Over the last years, insecticides less toxic and less persisting into mammalian organisms, such as pyrethroids, have been used. Some studies have indicated that these insecticides are highly active to nontarget aquatic organisms. Deltamethrin is a type II pyrethroid, is very toxic to crustaceans and fish even at low concentrations. It inhibits nicotinic acetylcholine receptors, channel voltage-dependent Ca+2 and channel voltage-dependent Na+2 of the neuronal membrane. Fish is very sensitive to many xenobiotics allowing their use as a way of evaluating environmental pollution. The organisms able to highlight biological responses to xenobiotics are said bioindicators. Their responses are the set of alterations in the homeostasis of the organism. Acetylcholinesterase (AChE) is a bioindicator able to hydrolyze acetylcholine into choline and acetate. In this project, that enzyme will be used to evaluate the effects of deltamethrin on the muscle of the teleost matrinxã Brycon cephalus. The fish will be exposed for 4, 24 e 48 hours to 0.26µg/L of the deltamethrin (10% of the LC;50) in a system of tanks "indoor". After this period, the fish will be anesthetized and killed to excision of white muscle and assay of AChE activity.(AU)