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Study of oxidative parameters in the central nervous system of obese rats supplemented chronically with green tea extract (Camellia Sinensis)

Grant number: 12/19681-5
Support type:Scholarships in Brazil - Master
Effective date (Start): April 01, 2013
Effective date (End): February 28, 2015
Field of knowledge:Biological Sciences - Physiology - General Physiology
Principal researcher:Rosemari Otton
Grantee:Rita de Cássia Macedo dos Santos
Home Institution: Pró-Reitoria de Pós-Graduação e Pesquisa. Universidade Cruzeiro do Sul (UNICSUL). São Paulo , SP, Brazil


Obesity is considered a chronic noncommunicable disease (CNCD) which is characterized by the accumulation of body fat that may impair health, encouraging the development of other diseases such as type 2 diabetes, cardiovascular disease, hypertension among others. It is well described that the brain is an organ that has a greater degree of oxidative stress as compared to other tissues, since it has a high oxygen consumption and high iron content, which promotes lipid peroxidation in addition to a decreased antioxidant defense capacity. Endoplasmic reticulum is an organelle responsible for synthesis of proteins, and a deficiency in this organelle can cause reticulum stress, mainly reported in diabetes mellitus and obesity. Catechins, the main polyphenolic compounds in green tea have multiple beneficial health effects because they have antioxidant effects, anti-hypertensive, anti-inflammatory, anti-proliferative, anti-thrombogenic, antimutagenic, antidiabetic and lipid lowering. Many studies have demonstrated the activity of several natural antioxidants in obesity and CNS, however, nothing is described on the antioxidant activity of green tea. Therefore, this study aims to evaluate the oxidative, inflammatory and functional parameters in different CNS regions (brain, brainstem and cerebellum) of obese animals treated with green tea extract. The following experiments will be carried out: lipids damage by measuring 8-isoprostane (ELISA assay), content of reduced glutathione (GSH), oxidized (GSSG) and GSH / GSSG ratio, evaluation of total antioxidant capacity of the brain regions by FRAP assay, the antioxidant enzymes SOD, glutathione reductase and peroxidase, plasma leptin level, gene expression of BIP, CHOP and XBP-1 as indicators of reticulum stress (RT-PCR) . (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MACEDO, RITA CASSIA; BONDAN, EDUARDO FERNANDES; OTTON, ROSEMARI. Redox status on different regions of the central nervous system of obese and lean rats treated with green tea extract. NUTRITIONAL NEUROSCIENCE, v. 22, n. 2, p. 119-131, . (11/19216-8, 12/19681-5)

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