Across the years modulation of cytochrome c-oxidase has been pointed as key-mechanism in interaction between LASER radiation with different biological tissues. More recently, a study demonstrated that cytochrome c-oxidase activity is increased by phototherapy in different types of skeletal muscle fibers of rats. Recent studies of our research group with phototherapy have shown positive results delaying skeletal muscle fatigue and improving the status of biochemical markers related to skeletal muscle recovery when these therapies were applied before exercise. We think that cytochrome c-oxidase activity can play a key role in low-level laser therapy (LLLT) effects in delaying skeletal muscle fatigue. With these perspectives in mind, this research project aims to evaluate effects of different LLLT doses and wavelengths in cytochrome c-oxidase activity in different types of skeletal muscle fibers, as well as to determine time interval that occurs peak of activity of cytochrome c-oxidase in different types of skeletal muscle fibers. For such, we will perform an animal experiment. In this experiment tibialis anterior muscle of rats will be irradiated with different LLLT doses (1, 3 and 10 J), with different wavelengths (660, 830 and 905 nm), with 50 mW power output. After irradiation muscles will be removed at different experimental times (5, 10, 30 minutes, 1, 2, 12 and 24 hours) and stored for further analysis. Analysis of cytochrome c-oxidase activity in different types of muscular fiber will be performed through immunohistochemical analysis, analysis of histological images, and Western Blotting.
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