Functional analyze of murine antibodies induced by immunization with a chimeric recombinant protein based on different immunodominant Plasmodium vivax antigens

• Grant number: 13/01487-0
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: April 01, 2013
• End date: June 30, 2013
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Principal Investigator: Irene da Silva Soares
• Grantee: Mariana Vilela Rocha
• Supervisor: Laurent Claude Stéphane Rénia
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Agency for Science, Technology and Research (A*STAR), Singapore
• Associated to the scholarship: 11/23278-9 - Generation of chimeric recombinant proteins for vaccine development against Plasmodium vivax, BP.IC

Abstract

Plasmodium vivax is the most world-widely distributed and the most prevalent specie in America. Vaccine development to P. vivax is considered a priority on the global program for the eradication of malaria. In our process linked entitled "Generation of chimeric recombinant proteins for vaccine development against Plasmodium vivax", we hypothesized that an effective vaccine formulation for a protective immune response should be composed by immunodominant regions of two antigens of the parasite. To test our hypothesis, we generate a chimeric protein based on the Apical Membrane Antigen 1 (AMA-1) and the C-terminal region of Merozoite Surface Protein 1 (MSP-1) that we previously characterized as highly immunogenic in natural infections. The chimeric recombinant protein (AMA166-MSP119) was recently expressed in yeast Pichia pastoris using synthetic codon optimized genes. Pre-clinical vaccinations in mice with this protein elicited high IgG antibody titres as detected by ELISA. The main objective of this project is evaluate if antibodies against AMA166-MSP119 are capable of recognize the native proteins present in mature isolated schizonts of P. vivax by immunofluorescence. Subsequently, our aim is investigate if antibodies induced by experimental immunizations are capable of inhibit reticulocyte invasion by P. vivax. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROCHA, MARIANA VILELA; FRANCOSO, KATIA SANCHES; LIMA, LUCIANA CHAGAS; CAMARGO, TARSILA MENDES; MACHADO, RICARDO L. D.; COSTA, FABIO T. M.; RENIA, LAURENT; NOSTEN, FRANCOIS; RUSSELL, BRUCE; RODRIGUES, MAURICIO M.; et al. Generation, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19). Vaccine, v. 35, n. 18, p. 2463-2472, APR 25 2017. (12/13032-5, 11/23278-9, 13/01487-0)