Identification of CNVs in the Nelore genome and its association with meat tenderness

• Grant number: 12/23717-5
• Support type: Scholarships in Brazil - Master
• Effective date (Start): April 01, 2013
• Effective date (End): January 31, 2015
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Genetics and Improvement of Domestic Animals
• Principal researcher: Luiz Lehmann Coutinho
• Grantee: Vinicius Henrique da Silva
• Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated scholarship(s): 14/01438-2 - Metabolic pathways and meat tenderness: from CNVs to gene expression in Nelore cattle breed, BE.EP.MS

Abstract

Brazil has the largest commercial cattle herd in the world, also taking the first place in the export of meat. Most of this herd consists of Zebu, whose the most prominent is the Nelore breed. The great adaptability against tropical environment of this breed, nevertheless, not comes with high meat tenderness. The purpose of this work is to construct a map of copy number variations (CNV) to Nelore, and describe that influence on the meat tenderness phenotype. This project will contribute to building a better understanding of the genetic architecture of the main Brazilian cattle breed. Will be used data from SNP chips (Illumina High-Density Bovine Beadchip ®) of approximately 800 animals from the project: "Strategies for genetic improvement of production efficiency and quality of beef in Brazil," conducted by EMBRAPA in collaboration with ESALQ . From parameters extracted from the chip the CNVs present in each animal will be estimated by the PennCNV software. The ten most statistically significant CNVs will be validated by qPCR (real-time quantitative polymerase chain reaction) methodology, in thirty bulls. The CNVs will be allocated with the genes and QTLs that overlap in the literature. Finally, the genes will be crossed with metabolic pathways that are involved.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, VINICIUS HENRIQUE; DE ALMEIDA REGITANO, LUCIANA CORREIA; GEISTLINGER, LUDWIG; PERTILLE, FABIO; GIACHETTO, POLIANA FERNANDA; BRASSALOTI, RICARDO AUGUSTO; MOROSINI, NATALIA SILVA; ZIMMER, RALF; COUTINHO, LUIZ LEHMANN. Genome-Wide Detection of CNVs and Their Association with Meat Tenderness in Nelore Cattle. PLoS One, v. 11, n. 6, JUN 27 2016. (12/23638-8, 14/01438-2, 12/23717-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Vinicius Henrique da. Identification of CNVs in the Nelore genome and its association with meat tenderness. 2015. Master's Dissertation - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.