"purification of proteases secreted by the psicrotroph yeast Rhodoturola mucilagionosa by aqueous two-phase systems (PEG/NaPA) and chromatographic processes"

• Grant number: 12/23726-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 2013
• End date: April 30, 2016
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Adalberto Pessoa Junior
• Grantee: Luciana Daniela Lario
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Proteases represent one of the three largest groups of industrial enzymes and account for about 60% of the total worldwide sales of enzymes. Although proteolytic enzymes can be obtained from animals and plants, microorganisms are the preferred source for industrial applications in view of the scientific and economical advantages. Among various groups of microbes, psychrotrophs, are ideal candidates for enzymes obtention, because enzymes isolated from those organisms are active at low temperatures and stable under alkaline conditions, in presence of oxidants and detergents. Those qualities are desirable in laundry industry, and are also useful for bioremediation, food technology and molecular biology. At this point, it should be stated, that isolation of biological products in large scale needs efficient techniques which can preserve the bio-products properties at a relatively low cost. Aqueous two phase systems (ATPSs) emerged as a powerful method to solve this problem. However, some products require a high degree of purity, in those cases, the extraction by ATPS is not enough, so it is necessary to add one or more chromatographic steps. The aim of this project is to set up an efficient method of purification of the protease secreted by the psicrotroph yeast Rhodoturola mucilagionosa by aqueous two-phase systems (PEG/NaPA) and chromatographic systems, to obtain more robust proteases of industrial and biotechnological significance. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CHAUD, LUCIANA C. S.; LARIO, LUCIANA D.; BONUGLI-SANTOS, RAFAELLA C.; SETTE, LARA D.; PESSOA JUNIOR, ADALBERTO; FELIPE, MARIA DAS GRACAS DE A.. Improvement in extracellular protease production by the marine antarctic yeast Rhodotorula mucilaginosa L7. NEW BIOTECHNOLOGY, v. 33, n. 6, p. 807-814, DEC 25 2016. (11/51796-4, 12/23726-4)

LARIO, LUCIANA DANIELA; PELLEGRINI MALPIEDI, LUCIANA; BRANDAO PEREIRA, JORGE FERNANDO; SETTE, LARA DURAES; PESSOA-JUNIOR, ADALBERTO. Liquid-liquid extraction of protease from cold-adapted yeast Rhodotorula mucilaginosa L7 using biocompatible and biodegradable aqueous two-phase systems. SEPARATION SCIENCE AND TECHNOLOGY, v. 51, n. 1, p. 57-67, JAN 2 2016. (13/19486-0, 11/20521-0, 12/23726-4)

MACHADO, SUELLEN; FEITOSA, VALKER; PILLACA-PULLO, OMAR; LARIO, LUCIANA; SETTE, LARA; PESSOA JR, ADALBERTO; ALVES, HARLEY. Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor. BIOENGINEERING-BASEL, v. 9, n. 11, p. 13-pg., 2022-11-01. (10/17033-0, 12/23726-4, 13/19486-0, 16/07957-7)

LARIO, LUCIANA DANIELA; CHAUD, LUCIANA; ALMEIDA, MARIA DAS GRACAS; CONVERTI, ATTILIO; DURAES SETTE, LARA; PESSOA, ADALBERTO. Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7. FUNGAL BIOLOGY, v. 119, n. 11, p. 1129-1136, NOV 2015. (13/19486-0, 12/23726-4)

VICENTE, FILIPA A.; LARIO, LUCIANA D.; PESSOA, JR., ADALBERTO; VENTURA, SONIA P. M.. Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants. Process Biochemistry, v. 51, n. 4, p. 528-534, APR 2016. (12/23726-4)