Chemical elucidation of biocompounds and molecular targets of geopropolis on cancer cell lines

• Grant number: 12/22002-2
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: June 03, 2013
• End date: June 02, 2014
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Pedro Luiz Rosalen
• Grantee: Marcos Guilherme da Cunha
• Supervisor: John A. Beutler
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Institution abroad: Center for Cancer Research (CCR), United States
• Associated to the scholarship: 11/23635-6 - Isolation and pharmacological evaluation of bioactive molecules from hexane fraction of M. scutellaris geopropolis, BP.DR

Abstract

Geopropolis is a resin collected by stingless bee with little description at literature. Recently, its biological properties have been studied by our research group and the antiproliferative was described as promising. As consequence, efforts have been made in order to achieve deep knowledge on chemical composition and further activities of this kind of propolis. Once the antiproliferative activity has shown being a promising field of study and the chemical content is not fully recognized, the aim of this study is to elucidate the chemical composition and the possible molecular targets of geopropolis and its isolate compounds on cancer cell lines, as part of original Ph.D. project (FAPESP #2011/23635-6). Thus, we have established a partnership with National Cancer Institute (Frederick, MD, USA) intending to perform the most advanced methodologies both in chemical structure elucidation and molecular targets in a worldwide reference center. Briefly, it will be used the follow methodologies: a) fractionation and purification of geopropolis and further chemical elucidation of bioactive compounds using nuclear magnetic resonance (NMR) experiments, including 1-dimensional experiments 1H and 13C and 2-dimensional experiments COSY, ROESY, HSQC, and HMBC and b) molecularly targeted assays will including p300/HIF-1 binding, MALT-1 protease enzyme inhibition, EpCAM/beta-catenin assay, p38 inhibition and inhibition of protein sumoylation (assays not available in Brazil). (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA CUNHA, MARCOS GUILHERME; ROSALEN, PEDRO LUIZ; FRANCHIN, MARCELO; DE ALENCAR, SEVERINO MATIAS; IKEGAKI, MASAHARU; RANSOM, TANYA; BEUTLER, JOHN ALBERT. Antiproliferative Constituents of Geopropolis from the Bee Melipona scutellaris. Planta Medica, v. 82, n. 3, p. 190-194, FEB 2016. (12/22002-2, 11/23635-6)

DA CUNHA, MARCOS GUILHERME; RAMOS-JUNIOR, ERIVAN SCHNAIDER; FRANCHIN, MARCELO; TAIRA, THAISE MAYUMI; BEUTLER, JOHN A.; NOBRE FRANCO, GILSON CESAR; IKEGAKI, MASAHARU; DE ALENCAR, SEVERINO MATIAS; FUKADA, SANDRA YASUYO; ROSALEN, PEDRO LUIZ. Effects of Cinnamoyloxy-mammeisin from Geopropolis on Osteoclast Differentiation and Porphyromonas gingivalis-Induced Periodontitis. Journal of Natural Products, v. 80, n. 6, p. 1893-1899, JUN 2017. (12/22002-2, 11/23635-6, 14/11958-3)

DA CUNHA, MARCOS GUILHERME; FRANCHIN, MARCELO; DE PAULA-EDUARDO, LAILA FACIN; FREIRES, IRLAN ALMEIDA; BEUTLER, JOHN A.; DE ALENCAR, SEVERINO MATIAS; IKEGAKI, MASAHARU; MACHADO TABCHOURY, CINTHIA PEREIRA; CUNHA, THIAGO MATTAR; ROSALEN, PEDRO LUIZ. Anti-inflammatory and anti-biofilm properties of ent-nemorosone from Brazilian geopropolis. Journal of Functional Foods, v. 26, p. 27-35, OCT 2016. (12/22002-2, 12/22378-2, 11/23635-6, 11/16501-3)

DA CUNHA, MARCOS GUILHERME; ORLANDI SARDI, JANAINA DE CASSIA; FREIRES, IRLAN ALMEIDA; FRANCHIN, MARCELO; ROSALEN, PEDRO LUIZ. Antimicrobial, anti-adherence and antibiofilm activity against Staphylococcus aureus of a 4-phenyl coumarin derivative isolated from Brazilian geopropolis. Microbial Pathogenesis, v. 139, FEB 2020. (11/23635-6, 12/22002-2)

DA CUNHA, MARCOS GUILHERME; NOBRE FRANCOB, GILSON CESAR; FRANCHIN, MARCELO; BEUTLER, JOHN A.; DE ALENCAR, SEVERINO MATIAS; IKEGAKI, MASAHARU; ROSALEN, PEDRO LUIZ. Prediction of pharmacokinetic and toxicological parameters of a 4-phenylcoumarin isolated from geopropolis: In silico and in vitro approaches. Toxicology Letters, v. 263, p. 6-10, NOV 30 2016. (12/22002-2, 11/23635-6)