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Determination of enzymes polyamine oxidase and peroxidase activity in Eucalyptus grandis x Eucalyptus urophylla

Grant number: 13/04678-1
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): June 01, 2013
Effective date (End): July 31, 2013
Field of knowledge:Biological Sciences - Botany - Pant Physiology
Principal researcher:Giuseppina Pace Pereira Lima
Grantee:Débora Zanoni Do Prado
Supervisor abroad: Fabio Vianello
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Research place: Università degli Studi di Padova, Italy  
Associated to the scholarship:12/14886-8 - Rooting of Eucalyptus grandis x Eucalyptus urophylla through the action of hydrogen peroxide and quercetin, BP.MS

Abstract

Vegetative propagation is the main form of Eucalyptus´s shoots production for several advantages, however some clones still have difficulties in propagation, inclusively in rooting. Auxins are characterized as the main growth regulator able to improve this process. Indirectly, flavonoids and hydrogen peroxide can also contribute for rooting by increasing the activity of endogenous auxin. The objective of this work is to establish relation between rooting and the activity of polyamine oxidase and peroxidase enzymes in Eucalyptus grandis x Eucalyptus urophylla. The enzyme activity will be determinated by spectrophotometric methods. Data will be submitted to variance analysis (ANOVA) and means will be compared by Tukey test at 5% probability.Keywords: polyamines, enzymes, peroxidase, biochemical markers. (AU)