Biofilm formation in different null mutants of Salmonella enterica

• Grant number: 12/25426-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2013
• End date: May 31, 2016
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Marcelo Brocchi
• Grantee: Bruna Leite Simioni
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

In Brazil, Salmonella Enteritidis emerged as a serious problem in poultry and public health as from 1993. The indiscriminate use of antibiotics in chickens caused an increased antimicrobial resistance in strains of human origin. The surveys carried out in 2001 continue showing the presence of S. Enteritidis in poultry materials as the main serovar responsible for human food infections. S. Enteritidis is able to form biofilms and survive in the environment of long-term farm and contaminate poultry and eggs. Various genes involved in biofilm formation, which express main and essential components of the biofilm, such as the extracellular matrix. Once established biofilms are difficult to eradicate, due to their high resistance compared to planktonic cells, and becoming a cause of serious problems in industry and public health. The most indicated procedure for the control of S. Enteritidis in poultry is the acquisition and production of lots free of the agent. The project aims to improve knowledge of biofilm formation in strains of S. Enteritidis, S. enterica Typhimurium and null mutants for ihfA, ihfB, ihfAB, fis, hupA, hupB, hupAB, hfq, rpoS, STM0341 and ssvA, investigating the importance of these genes in biofilm formation. It is expected that the results will contribute to a control of S. enterica.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

WERLE, CATIERINE HIRSCH; DAMIANI, IGOR; MILANEZ, GUILHERME PAIER; FARIAS, ALESSANDRO S.; CINTRA GOMES MARCONDES, MARIA CRISTINA; CULLER, HEBERT FABRICIO; SIRCILI, MARCELO PALMA; LEITE, BRUNA; BROCCHI, MARCELO. Antimelanoma effect of Salmonella Typhimurium integration host factor mutant in murine model. FUTURE ONCOLOGY, v. 12, n. 20, p. 2367-2378, OCT 2016. (12/25426-8, 12/05382-6, 14/13412-8)

LEITE, BRUNA; WERLE, CATIERINE HIRSCH; DO CARMO, CAMILA PINHEIRO; NOBREGA, DIEGO BORIN; MILANEZ, GUILHERME PAIER; CULLER, HEBERT FABRICIO; SIRCILI, MARCELO PALMA; ALVAREZ-MARTINEZ, CRISTINA E.; BROCCHI, MARCELO. Integration host factor is important for biofilm formation by Salmonella enterica Enteritidis. PATHOGENS AND DISEASE, v. 75, n. 6, AUG 2017. (12/10608-3, 12/25426-8, 12/05382-6)