Influence of steroid hormones in expression levels and location of ADAMTS-1, 4 and 9 in cells derived from ovarian tumors

• Grant number: 13/01092-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2013
• End date: April 30, 2015
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Vanessa Morais Freitas
• Grantee: Maíra de Assis Lima
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 10/07699-1 - Protease ADAMTS1 influencing breast cancer behavior and microenvironment , AP.JP

Abstract

Sex hormones modulate various activities in cancer, since influencing the process of tumorigenesis, cell growth and metastasis. The ADAMTS's (a disintegrin and metalloproteinase with Thrombospondin Thrombospondin-adamalysin or motifs) are secreted proteases and are involved in functions such as the processing of procollagen to collagen N-proteinase cleavage of the proteoglycan matrix and angiogenesis.Our aim is to evaluate whether the expression levels of ADAMTS 1, 4 and 9 (mRNA and protein) and its subcellular localization are altered in the presence of the sex hormones estrogen, progesterone and testosterone in cell lines derived from ovarian tumors with different degrees of malignancy , and verify the presence of these hormones strains increase or decrease its invasiveness. Strains NIH-OVCAR-3 and ES-2, and proliferation steps after proper preparation, will be treated with concentrations of the hormones progesterone, estrogen or testosterone. Levels of ADAMTS (mRNA) will be analyzed by qPCR. The values derived from fluorescence obtained by qPCR are statistically analyzed with ANOVA and differences are considered statistically significant for pd 0.05. The Nives of ADAMTS (protein), obtained from the lysate total, will be analyzed by immunoblotting. The values of the area of each of the bands are subjected to the same statistical analysis described above. To identify the subcellular proteases in cell lines will be used immunofluorescence technique. To assess whether steroid hormones lead to an increase or decrease in the invasiveness of ovarian cancer lines will be a test of invasion, where cells are placed in front of each hormone individually and this will be compared to a control sample not treated with hormones. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LIMA, MAIRA ASSIS; DOS SANTOS, LILIANE; TURRI, JOSE ANTONIO; NONOGAKI, SUELY; BUIM, MARCILEI; LIMA, JOEMA FELIPE; VIANA PINHEIRO, JOAO DE JESUS; BUENO DE TOLEDO OSORIO, CYNTHIA APARECIDA; SOARES, FERNANDO AUGUSTO; FREITAS, VANESSA MORALS. Prognostic Value of ADAMTS Proteases and Their Substrates in Epithelial Ovarian Cancer. PATHOBIOLOGY, v. 83, n. 6, p. 316-326, 2016. (13/01092-6, 10/07699-1)

SILVA, SUELY V.; LIMA, MAIRA A.; CELLA, NATHALIE; JAEGER, RUY G.; FREITAS, VANESSA M.. ADAMTS-1 Is Found in the Nuclei of Normal and Tumoral Breast Cells. PLoS One, v. 11, n. 10, OCT 20 2016. (13/01092-6, 15/02498-1, 15/09845-9, 12/10053-1, 10/07699-1, 15/19773-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LIMA, Maíra de Assis. Influence of steroid hormones in the migration, invasion and expression of ADAMTS 1 and 4 proteases in ovary cancer cells.. 2015. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.