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Evaluation of the technological potential of the crude enzyme extract produced by the thermophilic fungus Myceliophthora SP. f. 2.1.4 for hydrolysis of sugar cane bagasse: a comparison between enzyme production by submerged fermentation and solid state

Grant number: 13/02574-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2013
Effective date (End): December 31, 2013
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Gustavo Orlando Bonilla Rodriguez
Grantee:Marcello Bertoldi Sanchez Neves
Home Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

The great need for changes in the global energy system by fossil fuel depletion and environmental changes leads to the search for alternative sustainable energy, especially biofuels such as ethanol of second generation. The present study aims to evaluate the technological potential of the crude extract enzyme produced by the thermophilic fungus Myceliophthora sp., In bioethanol production through enzymatic hydrolysis of bagasse cane sugar. The fungus will be grown in solid state fermentation and submerged, containing sheredded cardboad as lignocellulosic substrate . The extracts will be characterized (optimum pH and temperature, thermal stability and related to pH and ion effect on enzyme activity) and the activity of ²-glucosidase and cellulase endoglicanase. Enzymatic hydrolysis of sugar cane bagasse will be conducted under conditions determined as optimal and by the determination of the sugar profile will be possible to compare the application potential of the extract obtained by each fermentation system. Furthermore the aim is to optimize the process of saccharification using different concentrations of nonionic surfactants such as Tween 20 and Tween 80.