Involvement of opioid neurotransmission of the medial amygadala in the mediation of autonomic and hormonal responses evoked by restraint stress in rats

• Grant number: 13/00249-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): May 01, 2013
• Effective date (End): June 30, 2016
• Field of knowledge: Biological Sciences - Pharmacology - Neuropsychopharmacology
• Principal Investigator: Fernando Morgan de Aguiar Correa
• Grantee: Aline Fassini
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Several structures of the central nervous system are involved in triggering stress responses through the release of neurotransmitters and neuromodulators specific. Among these structures, the amygdala is known for its role in cardiovascular, hormonal and behavioral control during aversive situations. The restraint stress is a standard model in our laboratory and has been used to evaluate autonomic changes, hormonal and behavioral disorders due to stress. The ER cause significant increase in neuronal activity of the medial nucleus of the amygdala (MeA) when compared to other amygdaloid nuclei. In addition, previous publications from our group suggest an inhibitory function of the MeA in tachycardiac response induced by ER, while the literature indicates its involvement in the hormonal release (oxytocin and corticosterone) observed during the ER. The opioid system participates of the mediating cardiovascular responses, including those associated with aversive situations. Furthermore, was showing the presence of peptides and opioid receptors in amygdala, suggesting the existence of a functional opioid neurotransmission that structure. Based on the facts mentioned above, the hypothesis of this project is that the MeA opioid neurotransmission is involved in the modulation of autonomic hormonal responses evoked by ER. Pilot experiment demonstrated the administration of naloxone in the MeA affects the cardiovascular response evoked by ER, indicating the viability of the project. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRASIL, TAIZ F. S.; FASSINI, ALINE; CORREA, FERNANDO M.. AT1 and AT2 Receptors in the Prelimbic Cortex Modulate the Cardiovascular Response Evoked by Acute Exposure to Restraint Stress in Rats. Cellular and Molecular Neurobiology, v. 38, n. 1, SI, p. 305-316, JAN 2018. (13/00249-9)

BUSNARDO, CRISTIANE; FASSINI, ALINE; RODRIGUES, BRUNO; ANTUNES-RODRIGUES, JOSE; CRESTANI, CARLOS C.; CORREA, FERNANDO M. A.. N-Methyl-D-aspartate Glutamate Receptor Modulates Cardiovascular and Neuroendocrine Responses Evoked by Hemorrhagic Shock in Rats. BIOMED RESEARCH INTERNATIONAL, v. 2021, AUG 13 2021. (13/00249-9, 21/00148-4, 18/04899-1)

BRASIL, TAIZ F. S.; FASSINI, ALINE; CORREA, FERNANDO M.. AT1 and AT2 Receptors in the Prelimbic Cortex Modulate the Cardiovascular Response Evoked by Acute Exposure to Restraint Stress in Rats. Cellular and Molecular Neurobiology, v. 38, n. 1, p. 12-pg., 2018-01-01. (13/00249-9)

BUSNARDO, C.; CRESTANI, C. C.; FASSINI, A.; RESSTEL, L. B. M.; CORREA, F. M. A.. NMDA AND NON-NMDA GLUTAMATE RECEPTORS IN THE PARAVENTRICULAR NUCLEUS OF THE HYPOTHALAMUS MODULATE DIFFERENT STAGES OF HEMORRHAGE-EVOKED CARDIOVASCULAR RESPONSES IN RATS. Neuroscience, v. 320, p. 149-159, APR 21 2016. (13/00249-9)

FASSINI, ALINE; SCOPINHO, AMERICA A.; RESSTEL, LEONARDO B. M.; CORREA, FERNANDO M. A.. kappa-Opioid receptors in the infralimbic cortex modulate the cardiovascular responses to acute stress. Experimental Physiology, v. 100, n. 4, p. 377-387, APR 2015. (12/17626-7, 13/00249-9, 12/09300-4)

FASSINI, ALINE; SCOPINHO, AMERICA A.; RESSTEL, LEONARDO B. M.; CORREA, FERNANDO M. A.. NOP receptors in the prelimbic cortex have an inhibitory influence on cardiovascular responses induced by restraint stress. Neuropeptides, v. 57, p. 35-44, JUN 2016. (13/00249-9, 13/13721-8)

FASSINI, ALINE; SCOPINHO, AMERICA A.; ALVES, FERNANDO H. F.; FORTALEZA, EDUARDO A. T.; CORREA, FERNANDO M. A.. THE MEDIAL PREOPTIC AREA MODULATES AUTONOMIC FUNCTION UNDER RESTING AND STRESS CONDITIONS. Neuroscience, v. 364, p. 164-174, NOV 19 2017. (13/00249-9, 12/18556-2, 13/13721-8)

FASSINI, ALINE; SCOPINHO, AMERICA A.; RESSTEL, LEONARDO B. M.; CORREA, FERNANDO M. A.. Opioid receptors in the prelimbic cortex modulate restraint stress-induced cardiovascular responses in the rat. Neuropharmacology, v. 85, p. 367-374, OCT 2014. (12/17626-7, 13/00249-9, 12/09300-4)

FASSINI, ALINE; RESSTEL, LEONARDO B. M.; CORREA, FERNANDO M. A.. Prelimbic cortex GABA(A) receptors are involved in the mediation of restraint stress-evoked cardiovascular responses. STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS, v. 19, n. 6, p. 576-584, 2016. (13/00249-9)

HARTMANN, ALICE; FASSINI, ALINE; SCOPINHO, AMERICA; CORREA, FERNANDO M. A.; GUIMARAES, FRANCISCO S.; LISBOA, SABRINA F.; RESSTEL, LEONARDO B. M.. Role of the endocannabinoid system in the dorsal hippocampus in the cardiovascular changes and delayed anxiety-like effect induced by acute restraint stress in rats. JOURNAL OF PSYCHOPHARMACOLOGY, v. 33, n. 5, p. 606-614, MAY 2019. (17/19731-6, 16/14282-6, 13/13721-8, 14/24828-0, 12/17626-7, 13/00249-9)