Role of maspin in cell migration and proliferation

• Grant number: 13/00815-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2013
• End date: October 31, 2017
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Nathalie Cella
• Grantee: Mariana Tamazato Longhi
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 15/16384-8 - Regulation of maspin phosphorylation by the EGFR pathway, BE.EP.DR

Abstract

Maspin (mammary serpin) is a 42 kDa protein which belongs to superfamily of serpins (serine protease inhibitor) due to sequence homology, however, its mechanism of action does not depend on protease inhibition as indicated by the adopted nomenclature. This protein was identified in 1994 during a search for genes expressed in normal mammary epithelial cells but absent in breast tumors. Only one maspin gene and maspin protein were described, despite the great diversity of biological activities, ligands and subcellular localizations of this protein. Several studies indicate that maspin is regulated by post-translational modification, yet, little is known about how these alterations regulate its biological activities and intracellular pathways. Our preliminary results indicate that EGF and signals induced by cell-substrate interaction regulate maspin post-translational modification. Maspin, EGF and extracellular matrix elements regulate two fundamental cellular processes which are altered in tumor progression: cell migration and proliferation. The objectives of this project are characterize maspin post-translational modifications, to investigate how EGF signal pathway interferes on these alterations and to investigate the role of maspin on cell migration and proliferation. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LONGHI, M. T.; SILVA, L. E.; PEREIRA, M.; MAGALHAES, M.; REINA, J.; VITORINO, F. N. L.; GUMBINER, B. M.; DA CUNHA, J. P. C.; CELLA, N.. PI3K-AKT, JAK2-STAT3 pathways and cell-cell contact regulate maspin subcellular localization. CELL COMMUNICATION AND SIGNALING, v. 19, n. 1, AUG 14 2021. (17/18344-9, 13/00815-4, 15/09309-0, 13/07467-1, 15/16384-8)

LONGHI, MARIANA TAMAZATO; MAGALHAES, MAGNA; REINA, JEFFREY; FREITAS, VANESSA MORAIS; CELLA, NATHALIE. EGFR Signaling Regulates Maspin/SerpinB5 Phosphorylation and Nuclear Localization in Mammary Epithelial Cells. PLoS One, v. 11, n. 7, JUL 22 2016. (13/00815-4, 15/02498-1, 10/07699-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LONGHI, Mariana Tamazato. Mechanisms of subcellular localization of maspin in breast epithelial cell line.. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.