Molecular phylogenetics of Mesembrinellidae (Diptera: Oestroidea): inference of among-genera and among-species relationships, divergence time estimations and biogeographical aspects

• Grant number: 12/23200-2
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2013
• End date: May 31, 2016
• Field of knowledge: Biological Sciences - Zoology - Taxonomy of Recent Groups
• Principal Investigator: Dalton de Souza Amorim
• Grantee: Marco Antonio Tonus Marinho
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The family Calliphoridae (Diptera: Oestroidea), in its traditional definition, probably comprises a very diverse and heterogeneous paraphyletic grouping of calyptrate dipterous. One of the reasons that render Calliphoridae a non-natural group is the inclusion of Mesembrinellidae, a group with exclusively Neotropical distribution, as one of its subfamilies. Recent studies have repeatedly showed that this clade should be treated with family staus, sister to Tachinidae. The diversity, biology and phylogenetic relationships of its subordinated groups are still poorly understood, there being no molecular phylogenetic studies available so far for the clade. In this context, this project aims to contribute to the available knowledge of this Oestroidea group, with a study of (1) the diversity and biogeographical distribution of Calliphoridae in the Neotropical region; and (2) the phylogenetic relationships among the subordinated groups of Mesembrinellidae. The material for the analyses will be obtained by field collections and by request to possible collaborative groups. For the analyses, the initial repertoire of molecular markers will comprise the ITS2, COI and COII regions, with the possibility of further inclusion of additional markers. Alignments will be conducted with the MAFFT and 4SALE programs and will include consistency tests through reiteration with changes in the order of taxa in the matrix using the initial obtained topologies. Molecular phylogenetic analyses will be conducted using the maximum-parsimony, maximum-likelihood and Bayesian inference methods. Divergence time estimations will also be conducted using Bayesian inference methods. The project also includes a particular study concerning the use of secondary structure information of ribosomal RNA molecules (continuing the work developed by the proponent in his PhD) as an additional source of phylogenetic data, homology testing and alignment. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARDOSO, GISELE ANTONIAZZI; DESZO, MARINA SANTOS; TORRES, TATIANA TEIXEIRA. Evolution of coding sequence and gene expression of blowflies and botflies with contrasting feeding habits. Genomics, v. 113, n. 1, 2, p. 699-706, JAN 2021. (16/09659-3, 14/13933-8, 12/23200-2, 14/01600-4)

JUNQUEIRA, ANA CAROLINA M.; AZEREDO-ESPIN, ANA MARIA L.; PAULO, DANIEL F.; MARINHO, MARCO ANTONIO T.; TOMSHO, LYNN P.; DRAUTZ-MOSES, DANIELA I.; PURBOJATI, RIKKY W.; RATAN, AAKROSH; SCHUSTER, STEPHAN C.. Large-scale mitogenomics enables insights into Schizophora (Diptera) radiation and population diversity. SCIENTIFIC REPORTS, v. 6, FEB 25 2016. (12/23200-2, 08/56769-2, 10/09961-5)

AMAT, EDUARDO; TONUS MARINHO, MARCO ANTONIO; RAFAEL, JOSE ALBERTINO. A survey of necrophagous blowflies (Diptera: Oestroidea) in the Amazonas-Negro interfluvial region (Brazilian Amazon). Revista Brasileira de Entomologia, v. 60, n. 1, p. 57-62, JAN-MAR 2016. (12/23200-2)

CARDOSO, GISELE ANTONIAZZI; DESZO, MARINA SANTOS; TORRES, TATIANA TEIXEIRA. Evolution of coding sequence and gene expression of blowflies and botflies with contrasting feeding habits. Genomics, v. 113, n. 1, p. 8-pg., 2021-01-25. (12/23200-2, 14/01600-4, 14/13933-8, 16/09659-3)

TONUS MARINHO, MARCO ANTONIO; WOLFF, MARTA; RAMOS-PASTRANA, YARDANY; LIMA DE AZEREDO-ESPIN, ANA MARIA; AMORIM, DALTON DE SOUZA. The first phylogenetic study of Mesembrinellidae (Diptera: Oestroidea) based on molecular data: clades and congruence with morphological characters. CLADISTICS, v. 33, n. 2, p. 134-152, APR 2017. (12/23200-2, 03/10274-9)

PAULA RAILE RICCARDI; DIEGO AGUILAR FACHIN; ROSALY ALE-ROCHA; EDNA MARIA AMARAL; DALTON DE SOUZA AMORIM; LEONARDO HENRIQUE GIL-AZEVEDO; RENATO SOARES CAPELLARI; DANIEL DIAS DORNELAS DO CARMO; CLAUDIO JOSÉ BARROS DE CARVALHO; GUSTAVO BORGES FERRO; et al. Checklist of the dipterofauna (Insecta) from Roraima, Brazil, with special reference to the Brazilian Ecological Station of Maracá. Papéis Avulsos de Zoologia (São Paulo), v. 62, 2022-05-16. (21/08713-2, 13/16524-9, 18/14504-4, 16/02475-4, 14/05793-1, 12/12843-0, 12/23200-2)

CERRETTI, PIERFILIPPO; STIREMAN, III, JOHN O.; PAPE, THOMAS; O'HARA, JAMES E.; MARINHO, MARCO A. T.; ROGNES, KNUT; GRIMALDI, DAVID A.. First fossil of an oestroid fly (Diptera: Calyptratae: Oestroidea) and the dating of oestroid divergences. PLoS One, v. 12, n. 8, AUG 23 2017. (12/23200-2)

CARDOSO, GISELE ANTONIAZZI; TONUS MARINHO, MARCO ANTONIO; MONFARDINI, RAQUEL DIETSCHE; LIMA DE AZEREDO ESPIN, ANA MARIA; TORRES, TATIANA TEIXEIRA. Evolution of genes involved in feeding preference and metabolic processes in Calliphoridae (Diptera: Calyptratae). PeerJ, v. 4, OCT 27 2016. (12/19987-7, 14/13933-8, 12/23200-2, 09/13463-3, 14/01600-4)

WOLFF, MARTA; RAMOS-PASTRANA, YARDANY; TONUS MARINHO, MARCO ANTONIO; AMORIM, DALTON DE SOUZA. Two new species of Huascaromusca Townsend from Colombia, and a new combination for Giovanella carvalhoi (Diptera: Mesembrinellidae). Zootaxa, v. 4231, n. 2, p. 251-263, FEB 10 2017. (12/23200-2)