Association between single nucleotide polymorphism related to adiponectin, Toll like receptor 4, IL-1² and IL-6 genes and fatty acids intake and their effects on systemic inflammatory pattern in a population based study - ISA capital.

• Grant number: 13/01740-8
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): June 01, 2013
• Effective date (End): May 31, 2015
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Marcelo Macedo Rogero
• Grantee: Marina Maintinguer Norde
• Host Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Since the late 1990s, an increase in sedentary behavior, in consumption of food rich in saturated, trans and omega-6 fatty acids, sugar and low in fiber have been found, as well as a decreased intake of fruits and vegetables, changes in feeding behavior and, as a consequence, increased prevalence of obesity in the population worldwide. Besides obesity, the quantity and quality of dietary fatty acids contributed to the emergence of a chronic low grade inflammation, related to the development of chronic non-communicable diseases. Studies in nutrigenetics aim to identify genetic variations that generate different metabolic responses to a certain type of dietary pattern in a population. Thus, genetic variations can interfere with the relationship between dietary fatty acids and inflammatory biomarkers. Accordingly, the present study aims to verify the association between single nucleotide polymorphisms (SNP) of adiponectin, Toll-like receptor (TLR)-4, Interleukine (IL)-1² and IL-6 genes and fatty acids intake and their effect over inflammatory biomarkers plasma concentrations in a transversal study - ISA Capital. Three hundred and three individuals will be studied, aged 20 to 59 years. The dietary data were collected by 24 hour food recall, applied in duplicate. These dietary data were transformed into energy and nutrients using the Nutrition Data System for Research (NDS-R, Minessota, USA) software. The home blood collection was performed by specialized professional, after fasting for 12 hours, with prior appointment. Out of plasma, IL-1², IL-6, IL-8, IL-10, tumoral necrosis factor (TNF)-±, IL-12p70, adiponectin, C-reactive protein, macrophage chemotactic protein (MCP)-1, soluble intracellular adhesion molecule (sICAM)-1 and soluble vascular adhesion molecule (sVCAM)-1 will be determined using the immunoassay multiplex technique. The genomic DNA will be extracted from the total blood using the salting out method and the SNP of of adiponectin (rs266729, rs17300539, rs16861209, rs1501299 e rs2241766), TLR4 (rs4986790 e rs5030728), Interleukine (IL)-1² (rs1143623, rs16944, rs1143627, rs1143634 e rs1143643) and IL-6 (rs1800795) will be genotyped by allele specific PCR.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OKI, ERICA; NORDE, MARINA N.; CARIOCA, ANTONIO A. F.; SOUZA, JOSE M. P.; CASTRO, INAR A.; MARCHIONI, DIRCE M. L.; FISBERG, REGINA M.; ROGERO, MARCELO M.. Polymorphisms of the TNF-alpha gene interact with plasma fatty acids on inflammatory biomarker profile: a population-based, cross-sectional study in Sao Paulo, Brazil. BRITISH JOURNAL OF NUTRITION, v. 117, n. 12, p. 1663-1673, JUN 2017. (14/16347-2, 12/20401-7, 13/01741-4, 13/01740-8, 09/15831-0)

OKI, ERICA; NORDE, MARINA M.; CARIOCA, ANTONIO A. F.; IKEDA, RENATA E.; SOUZA, JOSE M. P.; CASTRO, INAR A.; MARCHIONI, DIRCE M. L.; FISBERG, REGINA M.; ROGERO, MARCELO M.. Interaction of SNP in the CRP gene and plasma fatty acid profile in inflammatory pattern: A cross-sectional population-based study. NUTRITION, v. 32, n. 1, p. 88-94, JAN 2016. (12/20401-7, 13/01740-8, 09/15831-0, 13/01741-4)

DE SOUZA, WYSLLENNY N.; NORDE, MARINA M.; OKI, ERICA; ROGERO, MARCELO M.; MARCHIONI, DIRCE M. L.; FISBERG, REGINA M.; MARTINI, LIGIA A.. Association between 25-hydroxyvitamin D and inflammatory biomarker levels in a cross-sectional population-based study, Sao Paulo, Brazil. Nutrition Research, v. 36, n. 1, p. 1-8, JAN 2016. (12/20401-7, 13/01740-8, 13/01741-4)

NORDE, MARINA MAINTINGUER; OKI, ERICA; DE CASTRO, INAR ALVES; PACHECO SOUZA, JOSE MARIA; TEIXEIRA DAMASCENO, NAGILA RAQUEL; MARA FISBERG, REGINA; LOBO MARCHIONI, DIRCE MARIA; ROGERO, MARCELO MACEDO. Influence of adiponectin gene variants and plasma fatty acids on systemic inflammation state association-A cross-sectional population-based study, SAo Paulo, Brazil. MOLECULAR NUTRITION & FOOD RESEARCH, v. 60, n. 2, p. 278-286, FEB 2016. (12/20401-7, 13/01740-8, 09/15831-0, 13/01741-4)

NORDE, MARINA MAINTINGUER; OKI, ERICA; FERREIRA CARIOCA, ANTONIO AUGUSTO; TEIXEIRA DAMASCENO, NAGILA RAQUEL; FISBERG, REGINA MARA; LOBO MARCHIONI, DIRCE MARIA; ROGERO, MARCELO MACEDO. Influence of IL1B, IL6 and IL10 gene variants and plasma fatty acid interaction on metabolic syndrome risk in a cross-sectional population-based study. Clinical Nutrition, v. 37, n. 2, p. 659-666, APR 2018. (14/16347-2, 12/20401-7, 13/01741-4, 13/01740-8, 09/15831-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

NORDE, Marina Maintinguer. Association between single nucleotide plymorphisms in the genes of adiponectin, Toll like receptor-4, IL-1 and IL-6 and dietary fatty acids and their effects to a systemic inflammatory pattern at a population-based study ISA-Capital.. 2015. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Saúde Pública (FSP/CIR) São Paulo.