Association of dietary patterns and single nucleotide plymorphisms in the genes of adiponectin, Toll like receptor-4, IL-1², IL-6, IL-10, TNF-±, CCL-2 and C reactive protein with a systemic inflammatory pattern at a population-based study - ISA-Capital
Introduction: Experimental, epidemiological and clinical evidences point to a pathogenic role of inflammation on metabolic disorders development, being the dietary pattern an important risk factor for systemic low-grade inflammation. Single Nucleotide Polymorphisms (SNP) can modulate the relationship between diet and plasma inflammatory biomarkers levels. Objective: To verify the association of dietary patterns and SNP in the genes of adiponectin, Toll like receptor (TLR)-4, IL-1², IL-6, IL-10, tumor necrosis factor (TNF)-±, Chemokine C-C motif ligand (CCL)-2 and C reactive protein (CRP) with a systemic inflammatory pattern at a population-based study - ISA-Capital. Methods: This study sample will be composed by adults (20 to 59 years), participants of the population-based study ISA-capital 2008-2010 (n=302). Dietary data have been collected using two 24 hours dietary recall. Plasma concentration of adiponectin, CRP, IL-1², IL-6, IL-8, IL-10, TNF-±, IL-12p70, CCL-2, soluble intercellular adhesion molecule (sICAM)-1 and soluble vascular cell adhesion molecule (sVCAM)-1 was determined by multiplex immunoassay and a Cluster multivariate analysis (k-means) was conducted to separate individual according to their systemic inflammation. SNP from adiponectin (rs266729, rs17300539, rs16861209, rs1501299 e rs2241766), TLR4 (rs4986790, rs4686791, rs5030728, rs11536889), IL-1² (rs1143623, rs16944, rs1143627, rs1143634 e rs1143643) and IL-6 (rs1800795, rs1800796, rs1800797), IL-10 (rs1800871, rs1800872, rs1800896, rs3024490, rs1554286), TNF-± (rs1799964, rs 1799724, rs1800629, rs361525), CCL-2 (rs1024611) and CRP (rs1205, rs1417938, rs2808630) gene were genotyped by Taqman Open Array system. Dietary pattern analysis will be conducted using the Principal Component Analysis and scoring the diets using Brazilian Health Eating Index. Dietary variables will be tested according to its influence on systemic inflammatory patterns and on SNP or Genetic Risk Score and inflammation association.
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