Comparative Metallomics of transgenic and non-transgenic Arabidopsis thaliana´s leaves using 2D-HPLC-ICP-MS and ESI-MS/MS

• Grant number: 13/02810-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2013
• End date: May 31, 2015
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Marco Aurelio Zezzi Arruda
• Grantee: Katherine Chacón Madrid
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The objective of this research is to differentiate transgenic and non-transgenic Arabidopsis thaliana´s leaves in terms of seleno-proteomic. This work will use 2D-HPLC-ICP-MS/MS for quantification and identification of selenium in protein´s leaves. Initially, proteins of Arabidopsis thaliana´s leaves will be extracted by using phenol extraction method, thereafter, the protein mass will be determined from Bradford´s method. Chromatographic separations of protein will be developed using a size exclusion chromatography (SEC) as first dimension, and then a second dimension will be included by using anion exchange chromatography (AIX). The identification of seleno-proteins previously separated will be made by UPLC-ESI-MS/MS.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MADRID, Katherine Chacón. Study of selenium's effects relative to the production of reactive oxygen species and selenoproteins in genetically modified and unmodified Arabidopsis thaliana plants. 2015. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Química Campinas, SP.