| Grant number: | 13/00735-0 |
| Support Opportunities: | Scholarships in Brazil - Master |
| Start date: | July 01, 2013 |
| End date: | May 31, 2015 |
| Field of knowledge: | Biological Sciences - Morphology - Cytology and Cell Biology |
| Principal Investigator: | Silvya Stuchi Maria-Engler |
| Grantee: | Carolina Motter Catarino |
| Host Institution: | Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
Abstract Currently there is a strong global trend towards the development of in vitro tests that fulfill the use of animals in safety evaluation tests. In Brazil, this practice is still lacking, but should be implemented quickly so that meets international humanitarian concepts. Therefore, it is necessary a systematic methodology aimed at generating a test platform in epidermal equivalent, that when validated, meets the demands of the cosmetic and pharmaceutical industries, among others. The European Directive of 1986 led to the discussion of global reduction of animal testing and the 7th amendment of this Policy, published in 2004, ordered the transfer of the tests for risk assessment for in vitro system, and beyond, invested in alternative technologies. Different approaches have been developed to meet this need, for example, the generation of the epidermal equivalents, which was considered the most promising and widely studied. Two equivalents of epidermis (EpiDermTM Mattek-EPISKINTM and L'Oréal) commercially available were validated by ECVAM (European Centre for the Validation of Alternative Methods) and other models of reconstructed epidermis are being developed in different research laboratories in the world. These two models are recommended in the Guide 431 (Organisation for Economic Cooperation and Development - OECD) that evaluates the in vitro skin corrosion. Although the guide mention that the experiments can be performed with reconstructed skin in laboratories where these tests are conducted, the development of an epidermis equivalent as a kit will contribute to a standardized evaluation. Our laboratory has followed the global trend for the development of in vitro assays for assessing the risk of skin exposure to chemicals, with an emphasis on cosmetic and new molecules with therapeutic activity. Therefore, this project aims to create, within the principles of the Guide 431, an equivalent of epidermis and optimize this process through the incorporation of a biopolymer membrane (PET and collagen) and cellular support. In perspective, this epidermal equivalent may be used for research and risk assessment of pharmaceuticals, medicines, cosmetics, chemicals, pesticides, etc.., Contributing to the generation of alternative methods to animal testing in Brazil and entering the international trend of development tests for risk assessment. | |
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