The role of Th17 and Regulatory T cells (Treg) in immunomodulation of experimental periapical lesions

• Grant number: 13/05994-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): July 01, 2013
• Effective date (End): June 30, 2016
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Gustavo Pompermaier Garlet
• Grantee: Carolina Fávaro Francisconi Mortari
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The progression and severity of chronic periapical lesions are deeply influenced by host inflammatory and immune response, even dependent of presence of pathogenic microorganisms. In this context, different subtypes of lymphocytes, such as Th17 and Treg (regulatory T cells), have been implicated in the pathogenesis of such lesions. However, the role of Th17 and Tregs cells in the immunomodulation of chronic periapical lesions and its association have not been well known, as well as its the potential mechanisms involved. In this way, the aim of this project is investigate the role of Treg and Th17 in experimental periapical lesions in mice (C57BL/6) (Fukada, Silva et al. 2008; Garlet, Fukada et al. 2010; Spider Repeke et al. J Endond 2013) by histomorphometric and molecular analyses of the lesions in treating mice with anti-GITR (inhibition of Tregs, Garlet et al. 2010), IL-17 knockout animals (IL-17 KO with genetic background C57Bl/6) and untreated WT mice. For the histopathology and histomorphometric analysis, parameters as osteoclast number and pattern of mRNA expression (RealTimePCRarray) of factors involved in the destruction of soft tissue and in the process of osteoclastogenesis will be performed. The results of this project, involving the susceptibility or resistance of different lineages, will elucidate the role of Treg and Th17 cells in the development of periradicular diseases and the mechanisms involved in its regulation, besides it will improve the knowledge of the injury immunopathogenesis and the development of new strategies for diagnosis and clinical management of these pathologies. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FRANCISCONI, CAROLINA FAVARO; VIEIRA, ANDREIA ESPINDOLA; BIGUETTI, CLAUDIA CRISTINA; GLOWACKI, ANDREW J.; FAVARO TROMBONE, ANA PAULA; LETRA, ARIADNE; SILVA, RENATO MENEZES; SFEIR, CHARLES S.; LITTLE, STEVEN R.; GARLET, GUSTAVO POMPERMAIER. Characterization of the Protective Role of Regulatory T Cells in Experimental Periapical Lesion Development and Their Chemoattraction Manipulation as a Therapeutic Tool. JOURNAL OF ENDODONTICS, v. 42, n. 1, p. 120-126, JAN 2016. (12/15133-3, 13/05994-4)

ANA CLAUDIA ARAUJO-PIRES; CAROLINA FAVARO FRANCISCONI; CLAUDIA CRISTINA BIGUETTI; FRANCO CAVALLA; ANDREZA MARIA FABIO ARANHA; ARIADNE LETRA; ANA PAULA FAVARO TROMBONE; MARCELO FAVERI; RENATO MENEZES SILVA; GUSTAVO POMPERMAIER GARLET. Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status. Journal of Applied Oral Science, v. 22, n. 4, p. 336-346, 2014-07-00. (13/05994-4, 12/15133-3)

FRANCISCONI, C. F.; VIEIRA, A. E.; AZEVEDO, M. C. S.; TABANEZ, A. P.; FONSECA, A. C.; TROMBONE, A. P. F.; LETRA, A.; SILVA, R. M.; SFEIR, C. S.; LITTLE, S. R.; et al. RANKL Triggers Treg-Mediated Immunoregulation in Inflammatory Osteolysis. JOURNAL OF DENTAL RESEARCH, v. 97, n. 8, p. 917-927, JUL 2018. (13/05994-4, 12/15133-3, 15/24637-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MORTARI, Carolina Fávaro Francisconi. The role of Th17 and regulatory T cells (TREGs) in immunomodulation of experimental periapical lesions. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.