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The role of Th17 and Regulatory T cells (Treg) in immunomodulation of experimental periapical lesions

Grant number: 13/05994-4
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2013
Effective date (End): June 30, 2016
Field of knowledge:Biological Sciences - Immunology
Principal Investigator:Gustavo Pompermaier Garlet
Grantee:Carolina Fávaro Francisconi Mortari
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The progression and severity of chronic periapical lesions are deeply influenced by host inflammatory and immune response, even dependent of presence of pathogenic microorganisms. In this context, different subtypes of lymphocytes, such as Th17 and Treg (regulatory T cells), have been implicated in the pathogenesis of such lesions. However, the role of Th17 and Tregs cells in the immunomodulation of chronic periapical lesions and its association have not been well known, as well as its the potential mechanisms involved. In this way, the aim of this project is investigate the role of Treg and Th17 in experimental periapical lesions in mice (C57BL/6) (Fukada, Silva et al. 2008; Garlet, Fukada et al. 2010; Spider Repeke et al. J Endond 2013) by histomorphometric and molecular analyses of the lesions in treating mice with anti-GITR (inhibition of Tregs, Garlet et al. 2010), IL-17 knockout animals (IL-17 KO with genetic background C57Bl/6) and untreated WT mice. For the histopathology and histomorphometric analysis, parameters as osteoclast number and pattern of mRNA expression (RealTimePCRarray) of factors involved in the destruction of soft tissue and in the process of osteoclastogenesis will be performed. The results of this project, involving the susceptibility or resistance of different lineages, will elucidate the role of Treg and Th17 cells in the development of periradicular diseases and the mechanisms involved in its regulation, besides it will improve the knowledge of the injury immunopathogenesis and the development of new strategies for diagnosis and clinical management of these pathologies. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FRANCISCONI, C. F.; VIEIRA, A. E.; AZEVEDO, M. C. S.; TABANEZ, A. P.; FONSECA, A. C.; TROMBONE, A. P. F.; LETRA, A.; SILVA, R. M.; SFEIR, C. S.; LITTLE, S. R.; GARLET, G. P. RANKL Triggers Treg-Mediated Immunoregulation in Inflammatory Osteolysis. JOURNAL OF DENTAL RESEARCH, v. 97, n. 8, p. 917-927, JUL 2018. Web of Science Citations: 6.
FRANCISCONI, CAROLINA FAVARO; VIEIRA, ANDREIA ESPINDOLA; BIGUETTI, CLAUDIA CRISTINA; GLOWACKI, ANDREW J.; FAVARO TROMBONE, ANA PAULA; LETRA, ARIADNE; SILVA, RENATO MENEZES; SFEIR, CHARLES S.; LITTLE, STEVEN R.; GARLET, GUSTAVO POMPERMAIER. Characterization of the Protective Role of Regulatory T Cells in Experimental Periapical Lesion Development and Their Chemoattraction Manipulation as a Therapeutic Tool. JOURNAL OF ENDODONTICS, v. 42, n. 1, p. 120-126, JAN 2016. Web of Science Citations: 14.
ARAUJO-PIRES, ANA CLAUDIA; FRANCISCONI, CAROLINA FAVARO; BIGUETTI, CLAUDIA CRISTINA; CAVALLA, FRANCO; FABIO ARANHA, ANDREZA MARIA; LETRA, ARIADNE; FAVARO TROMBONE, ANA PAULA; FAVERI, MARCELO; SILVA, RENATO MENEZES; GARLET, GUSTAVO POMPERMAIER. Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status. Journal of Applied Oral Science, v. 22, n. 4, p. 336-346, Jul. 2014. Web of Science Citations: 49.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.