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Serological, molecular and images in patients with active lesions or acute relapse of ocular infection by Toxoplasma gondii: a study follow-up

Grant number: 13/10050-5
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2013
Effective date (End): July 31, 2014
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal Investigator:Cinara de Cássia Brandão
Grantee:Mariana Previato
Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil


Toxoplasmosis is an infection cosmopolitan with a small percentage of individuals by developing the clinical form. Constitutes a serious public health problem especially in women who have had no previous contact with Toxoplasma gondii in seropositive individuals who develop eye disease and others who have acquired some kind of immunosuppression. The aim of this project is to evaluate the use of serological methods, molecular and images in monitoring acute or acute deterioration of ocular toxoplasmosis. Specific objectives include: 1. select at least 10 consecutive patients attending the Outpatient-tively retinopathy eye injury with acute and/or reactivation of ocular injury resulting from infection by T. gondii 2. Identify classes of anti-T. gondii in patients with typical clinical ocular toxoplasmosis or acute flare-up 3. Identify the presence of genomic DNA of T. gondii in peripheral blood 4. Perform imaging examinations (fundus photography and OCT) 5. Evaluate the use of fundus photography and OCT during the healing process of the eye injury. 6. Compare the progress of healing with the results of serological analysis, and molecular images. Will be monitored for a period of one year, 10 patients in the Outpatient Hospital Retinopathy basic eye injury that present active/recurrent ocular toxoplasmosis. Will be correlated laboratory tests (serology and PCR), images (fundus photography and OCT) and clinical evaluation in order to establish a relationship with the evolution of the healing process of the eye injury. The data will be compared using Fisher's exact test or the chi-square test (if applicable) and t test (for continuous variables). Also, be calculated Odds Ratio values and confidence interval of 95%. The alpha value to be adopted is 5%. It is hoped that the results pos-sam contribute to the knowledge of the use of imaging tests in confirming the healing of eye injury by T. gondii. (AU)

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Scientific publications (10)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
AYO, CHRISTIANE MARIA; DA SILVEIRA CAMARGO, ANA VITORIA; FREDERICO, FABIO BATISTA; SIQUEIRA, RUBENS CAMARGO; PREVIATO, MARIANA; ANTUNES MURATA, FERNANDO HENRIQUE; SILVEIRA-CARVALHO, APARECIDA PERPETUO; BARBOSA, AMANDA PIRES; BRANDAO DE MATTOS, CINARA DE CASSIA; DE MATTOS, LUIZ CARLOS. MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis. PLoS One, v. 10, n. 12, . (13/15879-8, 13/25650-8, 13/06580-9, 13/10050-5, 09/17540-2, 15/13723-6)
AYO, CHRISTIANE MARIA; FREDERICO, FABIO BATISTA; SIQUEIRA, RUBENS CAMARGO; BRANDAO DE MATTOS, CINARA DE CASSIA; PREVIATO, MARIANA; BARBOSA, AMANDA PIRES; ANTUNES MURATA, FERNANDO HENRIQUE; SILVEIRA-CARVALHO, APARECIDA PERPETUO; DE MATTOS, LUIZ CARLOS. Ocular toxoplasmosis: susceptibility in respect to the genes encoding the KIR receptors and their HLA class I ligands. SCIENTIFIC REPORTS, v. 6, . (13/15879-8, 13/25650-8, 13/06580-9, 13/10050-5, 09/17540-2)
PREVIATO, M.; BORIM, M. P.; LIBERATORE, JR., R. D. R.; PIRES, A. C.; DIAS, M. A. F.; BRANDAO DE MATTOS, C. C.; DE MATTOS, L. C.. Lewis histo-blood group system phenotyping and genotyping reveal divergence in the association of Le(a-b-) phenotype and type 1 diabetes. Vox Sanguinis, v. 108, n. 3, p. 281-286, . (09/17540-2, 13/10050-5)
MEIRA-STREJEVITCH, CRISTINA SILVA; PEREIRA-CHIOCCOLA, VERA LUCIA; MAIA, MARTA MARQUES; CARNIETTO DE HIPPOLITO, DAISE DAMARIS; WANG, HUI-TZU LIN; MOTOIE, GABRIELA; DE SOUZA GOMES, APARECIDA HELENA; KANAMURA, CRISTINA TAKAMI; MARTINES, ROOSECELIS BRASIL; BRANDAO DE MATTOS, CINARA CASSIA; et al. Reference genes for studies in infectious parasitic diseases in five types of human tissues. GENE REPORTS, v. 7, p. 98-105, . (13/15879-8, 13/05811-7, 13/25650-8, 13/10050-5, 14/09496-1, 15/04803-6)
MURATA, FERNANDO HENRIQUE ANTUNES; PREVIATO, MARIANA; FREDERICO, FABIO BATISTA; BARBOSA, AMANDA PIRES; NAKASHIMA, FABIANA; DE FARIA, JR., GERALDO MAGELA; SILVEIRA CARVALHO, APARECIDA PERPETUO; MEIRA STREJEVITCH, CRISTINA DA SILVA; PEREIRA-CHIOCCOLA, VERA LUCIA; CASTIGLIONI, LILIAN; et al. Evaluation of Serological and Molecular Tests Used for the Identification of Toxoplasma gondii Infection in Patients Treated in an Ophthalmology Clinic of a Public Health Service in Sao Paulo State, Brazil. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 9, . (12/07716-9, 14/05302-8, 13/15879-8, 12/07750-2, 13/10050-5, 18/09448-8, 18/04709-8)
BRANDAO DE MATTOS, CINARA CASSIA; SIQUEIRA, RUBENS CAMARGO; FREDERICO, FABIO BATISTA; RIBEIRO FERREIRA, ISABELLE MARTINS; COSTA FERREIRA, ANA IARA; PREVIATO, MARIANA; PEREIRA-CHIOCCOLA, VERA LUCIA; DE MATTOS, LUIZ CARLOS. Toxoplasmic retinochoroiditis caused by Toxoplasma gondii strain ToxoDB#65. Acta Tropica, v. 185, p. 419-421, . (09/17540-2, 11/13939-8, 13/10050-5, 09/05505-8)
CAMILO, LILIAN MUNIZ; PEREIRA-CHIOCCOLA, VERA LUCIA; GAVA, RICARDO; MEIRA-STREJEVITCH, CRISTINA DA SILVA; VIDAL, JOSE ERNESTO; BRANDAO DE MATTOS, CINARA CASSIA; FREDERICO, FABIO BATISTA; DE MATTOS, LUIZ CARLOS; JUNQUEIRA FRANCO SPEGIORIN, LIGIA COSENTINO; ANTUNES MURATA, FERNANDO HENRIQUE; et al. Molecular diagnosis of symptomatic toxoplasmosis: a 9-year retrospective and prospective study in a referral laboratory in São Paulo, Brazil. Brazilian Journal of Infectious Diseases, v. 21, n. 6, p. 638-647, . (13/15879-8, 13/25650-8, 13/10050-5, 14/09496-1, 14/01706-7)
DE FARIA JUNIOR, GERALDO M.; AYO, CHRISTIANE M.; DE OLIVEIRA, AMANDA P.; LOPES, ALESSANDRO G.; FREDERICO, FABIO B.; SILVEIRA-CARVALHO, APARECIDA P.; PREVIATO, MARIANA; BARBOSA, AMANDA P.; MURATA, FERNANDO H. A.; DE ALMEIDA JUNIOR, GILDASIO CASTELLO; et al. CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis. Acta Tropica, v. 178, p. 276-280, . (13/10050-5, 09/17540-2, 13/15879-8, 15/17226-7)
MAIA, M. M.; MEIRA-STREJEVITCH, C. S.; PEREIRA-CHIOCCOLA, V. L.; DE HIPPOLITO, D. D. C.; SILVA, V. O.; BRANDAO DE MATTOS, C. C.; FREDERICO, F. B.; SIQUEIRA, R. C.; DE MATTOS, L. C.; GRP, FAMERP IAL TOXOPLASMA RES. Evaluation of gene expression levels for cytokines in ocular toxoplasmosis. PARASITE IMMUNOLOGY, v. 39, n. 10, . (13/15879-8, 13/25650-8, 13/10050-5, 14/09496-1, 15/04803-6)
MEIRA, CRISTINA S.; PEREIRA-CHIOCCOLA, VERA L.; VIDAL, JOSE E.; BRANDAO DE MATTOS, CINARA C.; MOTOIE, GABRIELA; COSTA-SILVA, THAIS A.; GAVA, RICARDO; FREDERICO, FABIO B.; DE MATTOS, LUIZ C.; GRP, TOXOPLASMA. Cerebral and ocular toxoplasmosis related with IFN-gamma, TNF-alpha, and IL-10 levels. FRONTIERS IN MICROBIOLOGY, v. 5, . (11/13939-8, 09/17540-2, 13/10050-5, 09/09168-6)

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