Microbial interactions between cariogenic bacteria: evaluation of growth, metabolic activity, biochemical composition and susceptibility to chlorhexidine of biofilms formed in vitro

• Grant number: 12/17236-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2013
• End date: March 31, 2016
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Fernanda Lourenção Brighenti
• Grantee: Rosa Virginia Dutra de Oliveira
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 14/02397-8 - Interactions between cariogenic bacteria: susceptibility to chlorhexidine and Confocal Scanning Laser Microscopy using drip-flow biofilm reactor, BE.EP.DR

Abstract

The proximity between microorganisms present in dental biofilm determines the existence of interaction between them, which can benefit the involved bacteria. This study aims to evaluate the interaction between Streptococcus mutans ATCC 35688 with two other cariogenic microorganisms: Actinomyces naeslundii ATCC 19039 and Streptococcus sobrinus ATCC 33478, in biofilms formed in vitro. First, biofilms growth will be standardized considering bacterial concentration (106, 107 and 108 UFC/ml) and growth period (24, 48 and 72 h). Biofilms will grow in triplicate on the surface of glass discs in BHI supplemented with 0.2% (v/v) sucrose, using a model of active attachment. The number of CFU/mL of each bacteria will be obtained with a stereomicroscope after plating in selective and/or differential culture media. Next, the metabolic activity (XTT test), the biochemical composition (biomass and production of proteins, carbohydrates and lactic acid) will be evaluated. To evaluate the susceptibility to chlorhexidine, biofilms will grow in a drip-flow reactor. The immediate effect of chlorhexidine and re-growth after 24 h will be assessed by confocal microscopy. Data of microbiology, metabolic and biochemical tests will be statistically analysed using the software GraphPad Prism version 3.02 considering the significance level of 5%. The confocal microscopy images will be analysed using COMSTAT. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DUTRA DE OLIVEIRA, ROSA VIRGINIA; SAMPAIO BONAFE, FERNANDA SALLOUME; PALOMARI SPOLIDORIO, DENISE MADALENA; KOGA-ITO, CRISTIANE YUMI; DE FARIAS, ALINE LEITE; KIRKER, KELLY R.; JAMES, GARTH A.; BRIGHENTI, FERNANDA LOURENCAO. Streptococcus mutans and Actinomyces naeslundii Interaction in Dual-Species Biofilm. MICROORGANISMS, v. 8, n. 2, FEB 2020. (12/17236-4, 14/02397-8, 13/12326-8)

DE FARIAS, ALINE LEITE; DE CARVALHO, LORENA PETRUCELLI FREIRE; MENDEZ, DANIELA ALEJANDRA CUSICANQUI; CRUVINEL, THIAGO; BRIGHENTI, FERNANDA LOURENCAO. Characterization of polymicrobial biofilms obtained from saliva or carious lesions in dentin. BIOFOULING, v. 36, n. 7, p. 11-pg., 2020-10-01. (14/02397-8, 13/12326-8, 17/25904-0, 12/17236-4)

ROSA VIRGINIA DUTRA DE OLIVEIRA; YASMIN ETIENNE ALBUQUERQUE; DENISE MADALENA PALOMARI SPOLIDORIO; CRISTIANE YUMI KOGA-ITO; ELISA MARIA APARECIDA GIRO; FERNANDA LOURENÇÃO BRIGHENTI. Efeito dos açucares da dieta em biofilme dupla espécie de Streptococcus mutans e Streptococcus sobrinus – um estudo piloto. Rev. odontol. UNESP, v. 45, n. 2, p. 90-96, 2016-02-12. (13/12326-8, 12/17236-4)

ROSA VIRGINIA DUTRA DE OLIVEIRA; YASMIN ETIENNE ALBUQUERQUE; DENISE MADALENA PALOMARI SPOLIDORIO; CRISTIANE YUMI KOGA-ITO; ELISA MARIA APARECIDA GIRO; FERNANDA LOURENÇÃO BRIGHENTI. Effect of dietary sugars on dual-species biofilms of Streptococcus mutans and Streptococcus sobrinus – a pilot study. Rev. odontol. UNESP, n. ahead, p. 0-0, 2016. (12/17236-4, 13/12326-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Rosa Virginia Dutra de. Interactions among cariogenic bacteria. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.