Evaluation of off-line and online microextraction for sample preparation of aqueous complex matrix containing fluoroquinolones and analysis by capillary liquid chromatography

• Grant number: 13/06294-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2013
• End date: September 30, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Alvaro José dos Santos Neto
• Grantee: Scarlet Nere Ortega
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

The occurrence of pharmaceutical products in the aquatic environment has increased the concern over the potential impact to the aquatic biota. In particular broad activity spectrum antibiotics, as fluoroquinolones (FQ), which possibly contribute to proliferation of antibiotic resistant bacteria. Due to the concern to the presence, persistence and fate of these products in environment, it has increased the development of very sensitive analytical methods suitable for the monitoring of antibiotics in trace levels. Many procedures published in the existing literature for analysis of FQs in environmental samples use bidimensional chromatography, column switching, in conventional scale. Besides, not much attention has been paid to MIP and RAM-MIP materials for fully automated and miniaturized configuration of SPE coupled to capillary liquid chromatography (cLC). Therefore, this work aims the development and evaluation of capillary columns packed with RAM-MIP sorbent for analysis of complex matrix containing fluoroquinolones, by SPE column switching coupled to cLC. This miniaturized approach will minimize time and analyses expenses and can contribute for the field development.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ORTEGA, S. N.; SANTOS-NETO, A. J.; LANCAS, F. M.. Development and optimization of a fast method for the determination of statins in human plasma using microextraction by packed sorbent (MEPS) followed by ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). ANALYTICAL METHODS, v. 9, n. 20, p. 3039-3048, MAY 28 2017. (13/06294-6, 10/19910-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ORTEGA, Scarlet Nere. Synthesis and evaluation of selective sorbents MIP and RAMIP for the analysis of fluoroquinolones in environmental complex matrix by column-switching LC-MS/MS. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Química de São Carlos (IQSC/BT) São Carlos.