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Genomic library of Passiflora edulis f. flavicarpa: exploitation by sequencing of BAC-ends and sequencing of candidate (gene-rich) BACs

Grant number: 13/11196-3
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): November 01, 2013
Effective date (End): January 31, 2017
Field of knowledge:Biological Sciences - Genetics - Plant Genetics
Principal Investigator:Maria Lúcia Carneiro Vieira
Grantee:Carla de Freitas Munhoz
Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Associated scholarship(s):16/00767-8 - Exploiting a genomic library of Passiflora edulis: BACs screening and PacBio sequencing, BE.EP.PD

Abstract

The yellow passion fruit (Passiflora edulis f. flavicarpa) is of considerable economic importance to Brazil. It is used to produce juice concentrate and also marketed for consumption as a fresh fruit. In the pharmaceutical industry, it is used to produce passiflora extract. Our research group has been working to generate a body of theoretical knowledge about the commercial species of Passiflora, investigating segregating populations to construct linkage maps and mapping quantitative loci based on their phenotypic and molecular characterization. However, very few are known about the composition and organization of the passion fruit genome. Recently, in collaboration with the INRA Toulouse, France, we provide a construction of a genomic library of P. edulis f. flavicarpa, inserted into BACs (Ped-B-Flav). This library contains 82,944 clones, giving coverage of about 6 X the genome of the species. As a way to extend the existing knowledge about the species, we intend to continue the BAC-ends sequencing and comparative genomic mapping, and also perform the complete sequencing of some candidate (gene-rich) BACs, aiming to shed light on this genome still unclear, making possible to identify a large number of protein coding regions, candidates for novel molecular markers and transposable elements, and get an idea of its organization. In a recent work, we identified some candidates BACs for complete sequencing, due to its collinearity with part of the genomes of Arabidopsis thaliana, Populus trichocarpa and Vitis vinifera. We hope that this study will take a big leap from what is known about the genome of Passiflora, and also make available to the scientific community tools that will facilitate the development of new theoretical and applied knowledge for crop improvement.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MUNHOZ, CARLA FREITAS; COSTA, ZIRLANE PORTUGAL; CAUZ-SANTOS, LUIZ AUGUSTO; EGOAVIL REATEGUI, ALINA CARMEN; RODDE, NATHALIE; CAUET, STEPHANE; DORNELAS, MARCELO CARNIER; LEROY, PHILIPPE; VARANI, ALESSANDRO DE MELLO; BERGES, HELENE; et al. A gene-rich fraction analysis of the Passiflora edulis genome reveals highly conserved microsyntenic regions with two related Malpighiales species. SCIENTIFIC REPORTS, v. 8, . (17/04216-9, 14/25215-2, 13/11196-3)

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