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Genetic diversity, genomics and phylogeography of manioc (Manihot esculenta Crantz): implications for the dispersion of the crop along the main fluvial axes in Brazilian Amazon Basin

Grant number: 13/11137-7
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2013
Effective date (End): July 31, 2015
Field of knowledge:Biological Sciences - Genetics
Principal Investigator:Maria Imaculada Zucchi
Grantee:Alessandro Alves Pereira
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

Manioc (Manihot esculenta ssp. esculenta Crantz) was domesticated in the southwestern Amazon basin and currently is the main source of calories for more than 800 million people in the world. After its initial domestication, divergent selective pressures gave rise to two major groups of varieties (sweet and bitter manioc), which differ in their contents of toxic compounds. Although there is some overlap, these groups of varieties have distinct patterns of distribution, which may be the outcome of limited processes of contact and interchange between varieties along the crop's domestication history. The evolutionary history of a crop may be better understood by studying the organization of genetic diversity within and between its domesticated populations. Few studies evaluated how the genetic diversity of manioc is organized across its geographic distribution, and there are no genomic population studies with manioc varieties. The main goal of this proposal is to evaluate, with different molecular markers, the genomic and genetic diversity and structure, and the phylogeography of manioc varieties traditionally grown along the major rivers of Brazilian Amazon basin. The current genetic diversity and structure will be evaluated with nuclear microsatellites markers (ncSSR), while historical patterns of the organization of genetic diversity (phylogeography) will be evaluated with chloroplastidial microsatellites (cpSSR). Novel information on the genomic basis for distinction between sweet and bitter varieties, as well as possible distinct patterns of dispersion, will be generated with SNPs detected with Restriction-site associated DNA sequencing (RAD-seq). We expect to contribute to the understanding of manioc's dispersal across the Amazon basin after its initial domestication, as well as to the understanding of the genomic basis for the differentiation between sweet and bitter manioc. This information may help identify centers of genetic diversity of the crop, which in turn may be useful for the conservation of genetic resources, and also for manioc's breeding programs.

Articles published in Agência FAPESP about the scholarship:
Most popular form of manioc has been consumed for 9,000 years 

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ZUCCHI, MARIA I.; SUJII, PATRICIA S.; MORI, GUSTAVO M.; VIANA, JOAO P. G.; GRANDO, CAROLINA; SILVESTRE, ELLIDA DE AGUIAR; SCHWARCZ, KAISER D.; MACRINI, CAMILA M.; BAJAY, MIKLOS M.; ARAUJO, FABIANO L.; SIQUEIRA, MARCOS V. B. M.; ALVES-PEREIRA, ALESSANDRO; DE SOUZA, ANETE P.; PINHEIRO, JOSE B.; RODRIGUES, RICARDO R.; BRANCALION, PEDRO H. S. Genetic diversity of reintroduced tree populations in restoration plantations of the Brazilian Atlantic Forest. RESTORATION ECOLOGY, v. 26, n. 4, p. 694-701, JUL 2018. Web of Science Citations: 1.
ALVES-PEREIRA, ALESSANDRO; CLEMENT, CHARLES R.; PICANCO-RODRIGUES, DORIANE; VEASEY, ELIZABETH A.; DEQUIGIOVANNI, GABRIEL; RAMOS, SANTIAGO L. F.; PINHEIRO, JOSE B.; ZUCCHI, MARIA I. Patterns of nuclear and chloroplast genetic diversity and structure of manioc along major Brazilian Amazonian rivers. ANNALS OF BOTANY, v. 121, n. 4, p. 625-639, MAR 2018. Web of Science Citations: 2.
SCHWARCZ, KAISER DIAS; SILVESTRE, ELLIDA DE AGUIAR; DE CAMPOS, JAQUELINE BUENO; SUJII, PATRICIA SANAE; GRANDO, CAROLINA; TRINDADE MACRINI, CAMILA MENEZES; DE SOUZA, ANETE PEREIRA; PINHEIRO, JOSE BALDIN; SANTIN BRANCALION, PEDRO HENRIQUE; RODRIGUES, RICARDO RIBEIRO; ZUCCHI, MARIA IMACULADA. Shelter from the storm: Restored populations of the neotropical tree Myroxylon peruiferum are as genetically diverse as those from conserved remnants. FOREST ECOLOGY AND MANAGEMENT, v. 410, p. 95-103, FEB 15 2018. Web of Science Citations: 0.
TAMBARUSSI, E. V.; SEBBENN, A. M.; ALVES-PEREIRA, A.; VENCOVSKY, R.; CAMBUIM, J.; DA SILVA, A. M.; DE MORAES, M. A.; DE MORAES, M. L. T. Dipteryx alata Vogel (Fabaceae), a neotropical tree with high levels of selfing: implications for conservation and breeding programs. ANNALS OF FOREST RESEARCH, v. 60, n. 2, p. 243-261, 2017. Web of Science Citations: 4.
MOREIRA, MILENE; ZUCCHI, MARIA I.; GOMES, JOSE E.; TSAI, SIU M.; ALVES-PEREIRA, ALESSANDRO; CARDOSO, ELKE J. B. N. Araucaria angustifolia Aboveground Roots Presented High Arbuscular Mycorrhizal Fungal Colonization and Diversity in the Brazilian Atlantic Forest. Pedosphere, v. 26, n. 4, p. 561-566, AUG 2016. Web of Science Citations: 3.
LEITE, N. A.; CORREA, A. S.; ALVES-PEREIRA, A.; CAMPOS, J. B.; ZUCCHI, M. I.; OMOTO, C. Cross-species amplification and polymorphism of microsatellite loci in Helicoverpa armigera and Helicoverpa zea (Lepidoptera: Noctuidae) in Brazilian cropping systems. Genetics and Molecular Research, v. 15, n. 2 2016. Web of Science Citations: 3.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.
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