Manioc (Manihot esculenta Crantz) is the staple food crop that originated in Amazonia that reached the biggest importance in the world, and is currently the eight most cultivated crop. Cultivated manioc (ssp. esculenta) was domesticated from M. esculenta ssp. flabellifolia, and currently two major groups of varieties (sweet and bitter manioc) are cultivated. The objective of this proposal is generating novel genomic resources for manioc through the whole genome re-sequencing of accessions of wild manioc using next-generation sequencing (NGS) technologies. Additionally, we intend to evaluate the molecular variation of genes with signatures of selection among cultivated manioc and the wild ancestor. Using target-sequencing, SNP markers will be identified in coding and non-coding portions of these genes to characterize functional genetic variation. New allelic and structural variants may be detected increasing the knowledge about the species' biology. The functional characterization these genes may evidence which cellular processes may have been affected during the evolution of the crop. Furthermore, whole genome re-sequencing will facilitate molecular marker discovery that may be used in genetic mapping applied to the crop's breeding.
News published in Agência FAPESP Newsletter about the scholarship: