Phenotypic and genotypic characterization of strains of Salmonella enterica isolated from pork in São Paulo.

• Grant number: 13/16946-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2013
• End date: February 28, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Andrea Micke Moreno
• Grantee: Vasco Túlio de Moura Gomes
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Currently, salmonellosis is one of the most important zoonoses on public health in the world, due to the high endemicity, mortality, and difficulty of control. In Brazil, several studies reported high frequency pigs eliminating agent in different stages of the production system. In São Paulo, you can find different realities when it comes to good manufacturing practices and quality control of products of animal origin, especially when one considers the points of sale to the consumer. Contamination by agents of foodborne infections is present and has been poorly evaluated in these environments. This study is part of an international collaboration involving the North Carolina State University and the University of Surrey to act as partners in the realization of part of the proposed tests and allow the training and knowledge exchange between his research group and the student responsible for the project. The main objectives are to assess the presence of Salmonella enterica in pork cuts sold in local markets, butchers and small markets distributed in five regions of São Paulo. The isolated strains will be characterized according to serotype, antimicrobial resistance profile, genotypic profile by pulsed field gel electrophoresis (PFGE) and typing by sequencing multiple sites (multilocus sequence typing - MLST). The data will be compared to the profiles of strains originating from swine farms and food poisoning cases reported in the country

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MATAJIRA, CARLOS E. C.; MORENO, LUISA Z.; GOMES, VASCO T. M.; SILVA, ANA PAULA S.; MESQUITA, RENAN E.; AMIGO, CRISTINA R.; CHRIST, ANA PAULA G.; SATO, MARIA INES Z.; MORENO, ANDREA M.. Molecular and antimicrobial susceptibility characterization of Globicatella sulfidifaciens isolated from sow's urinary tract infection. VETERINARY QUARTERLY, v. 37, n. 1, p. 48-51, 2017. (13/17136-2, 13/16946-0, 15/26159-1)

MORENO, LUISA Z.; MATAJIRA, CARLOS E. C.; GOMES, VASCO T. M.; SILVA, ANA PAULA S.; MESQUITA, RENAN E.; CHRIST, ANA PAULA G.; SATO, MARIA INES Z.; MORENO, ANDREA M.. Molecular and antibiotic susceptibility characterization of Aerococcus viridans isolated from porcine urinary infection. Veterinary Microbiology, v. 184, p. 7-10, FEB 29 2016. (13/17136-2, 13/16946-0)

MORENO, LUISA Z.; DA COSTA, BARBARA L. P.; MATAJIRA, CARLOS E. C.; GOMES, VASCO T. M.; MESQUITA, RENAN E.; SILVA, ANA PAULA S.; MORENO, ANDREA M.. Molecular and antimicrobial susceptibility profiling of Streptococcus dysgalactiae isolated from swine. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 86, n. 2, p. 178-180, OCT 2016. (15/26159-1, 13/17136-2, 13/16946-0)

MORENO, LUISA Z.; MATAJIRA, CARLOS E. C.; DA COSTA, BARBARA L. P.; FERREIRA, THAIS S. P.; SILVA, GIVAGO F. R.; DUTRA, MAURICIO C.; GOMES, VASCO T. M.; SILVA, ANA PAULA S.; CHRIST, ANA PAULA G.; SATO, MARIA INES Z.; et al. Characterization of porcine Trueperella pyogenes by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), molecular typing and antimicrobial susceptibility profiling in Sao Paulo State. COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, v. 51, p. 49-53, APR 2017. (15/26159-1, 13/17136-2, 13/16946-0)

MORENO, LUISA Z.; COMES, VASCO T. M.; MOREIRA, JESSICA; DE OLIVEIRA, CAROLINA H.; PERES, BARBARA P.; SILVA, ANA PAULA S.; THAKUR, SIDDHARTHA; LA RAGIONE, ROBERTO M.; MORENO, ANDREA M.. First report of mcr-1-harboring Salmonella enterica serovar Schwarzengrund isolated from poultry meat in Brazil. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 93, n. 4, p. 376-379, APR 2019. (16/25745-7, 13/16946-0)

MATAJIRA, CARLOS E. C.; MORENO, LUISA Z.; GOMES, VASCO T. M.; SILVA, ANA PAULA S.; MESQUITA, RENAN E.; DOTO, DANIELA S.; CALDERARO, FRANCO F.; DE SOUZA, FERNANDO N.; CHRIST, ANA PAULA G.; SATO, MARIA INES Z.; et al. Evaluation of protein spectra cluster analysis for Streptococcus spp. identification from various swine clinical samples. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, v. 29, n. 2, p. 245-249, MAR 2017. (15/26159-1, 13/17136-2, 13/16946-0)

SPINDOLA, MARIA G.; CUNHA, MARCOS P. V.; MORENO, LUISA Z.; AMIGO, CRISTINA R.; SILVA, ANA P. S.; PARRA, BEATRIZ M.; POOR, ANDRE P.; DE OLIVEIRA, CAROLINA H.; PEREZ, BARBARA P.; KNOBL, TEREZINHA; et al. Genetic diversity, virulence genotype and antimicrobial resistance of uropathogenic Escherichia coli (UPEC) isolated from sows. VETERINARY QUARTERLY, v. 38, n. 1, p. 79-87, OCT 26 2018. (13/17136-2, 13/16946-0, 14/11523-7, 16/25745-7, 17/09515-4, 11/22608-5)

MORENO, ANDREA MICKE; MORENO, LUISA ZANOLLI; POOR, ANDRE PEGORARO; MATAJIRA, CARLOS EMILIO CABRERA; MORENO, MARINA; DE MOURA GOMES, VASCO TULIO; RIBEIRO DA SILVA, GIVAGO FARIA; TAKEUTI, KARINE LUDWIG; BARCELLOS, DAVID EMILIO. Antimicrobial Resistance Profile of Staphylococcus hyicus Strains Isolated from Brazilian Swine Herds. ANTIBIOTICS-BASEL, v. 11, n. 2, p. 12-pg., 2022-02-01. (11/08541-5, 13/17136-2, 15/26159-1, 13/16946-0)

POOR, ANDRE P.; MORENO, LUISA Z.; MATAJIRA, CARLOS E. C.; PARRA, BEATRIZ M.; GOMES, VASCO T. M.; SILVA, ANA PAULA S.; DUTRA, MAURICIO C.; CHRIST, ANA PAULA G.; BARBOSA, MIKAELA R. F.; SATO, MARIA INES Z.; et al. Characterization of Corynebacterium diphtheriae, C. confusum and C-amycolatum isolated from sows with genitourinary infection. Veterinary Microbiology, v. 207, p. 149-152, AUG 2017. (15/26159-1, 15/26412-9, 13/17136-2, 13/16946-0)

MORENO, LUISA Z.; MATAJIRA, CARLOS E. C.; GOMES, VASCO T. M.; SILVA, ANA PAULA S.; MESQUITA, RENAN E.; CHRIST, ANA PAULA G.; SATO, MARIA INES Z.; MORENO, ANDREA M.. Molecular and antimicrobial susceptibility profiling of atypical Streptococcus species from porcine clinical specimens. INFECTION GENETICS AND EVOLUTION, v. 44, p. 376-381, OCT 2016. (15/26159-1, 13/17136-2, 13/16946-0)

GOMES, VASCO T. M.; MORENO, LUISA Z.; SILVA, ANA PAULA S.; THAKUR, SIDDHARTHA; LA RAGIONE, ROBERTO M.; MATHER, ALISON E.; MORENO, ANDREA M.. Characterization of Salmonella enterica Contamination in Pork and Poultry Meat from Sao Paulo/Brazil: Serotypes, Genotypes and Antimicrobial Resistance Profiles. PATHOGENS, v. 11, n. 3, p. 13-pg., 2022-03-01. (16/25745-7, 18/21216-5, 13/16946-0, 17/50453-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GOMES, Vasco Túlio de Moura. Phenotypic and genotypic characterization of strains of Salmonella enterica isolated from pork in São Paulo city. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.