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Production of rhamnolipids by mutants from Pseudomonas aeruginosa (rhlG and 2 A1)

Grant number: 13/20279-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2013
Effective date (End): April 30, 2016
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal researcher:Roberta Barros Lovaglio
Grantee:Graziela de Oliveira Teixeira Balieiro
Home Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil

Abstract

Despite the numerous advantages of biosurfactants, such as low toxicity, biodegradability and high stability, these compounds are not widely used because of the high cost of production. The application of alternative carbon sources can contribute to the reduction of production costs and increase the commercial use of biosurfactants. Furthermore, the understanding of genes involved in the biosynthesis of surfactant assists in understanding the development of tools entails an increase in the production of rhamnolipids. Therefore the present study aims to study the relationship between gene rhlG with the production of rhamnolipids and also assess whether there are differences in the metabolism of carbon sources on hydrophilic and hydrophobic deletion mutants in the gene above. Another point to be evaluated is the potential of biodiesel glycerol as a carbon source for the production of this surfactant by P. aeruginosa LBI 2A1.

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