Epigenetic reprogramming of canine embryonic germ cells

• Grant number: 13/10542-5
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): December 01, 2013
• Effective date (End): January 31, 2017
• Field of knowledge: Biological Sciences - Morphology
• Principal researcher: Daniele dos Santos Martins
• Grantee: Aline Fernanda de Souza
• Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated scholarship(s): 14/26761-0 - Reprogramming human embryonic germ cells, BE.EP.DR

Abstract

The epigenetic reprogramation is crucial for genome regulation during the development of germ cells, which are responsible for originating the gametogenic lineage in mammals. The demethylation in primordial germ cells is a unique key event to erase the epigenetic memory and prevent epimutations transmissions in the next generation. There is limited information on how these events are conserved in mammals, especially in dogs. The domestic dog (Canis lupus familiaris) proves to be an ideal model for the study of development in mammals despites numerous similarities with human physiology and biochemistry. The aim of this project is to study the epigenetic dinamic of embryonic germ cells since its initial development to migration and formation of genital ridge through DNA methylation and histone acetylation patterns. In a first stage, it will be held the derivation of possible canine embryonic germ cells during periods of 20, 23, 26 and 29 days of gestation. Cells will be cultured in vitro and characterized the morphology, immunophenotype, specific markers and cell viability. When evidenced the time window in which the potential germ cells will be successfully derived and grown, epigenetic analyzes of DNA methylation and histone acetylation will be performed to characterize the time-dependent epigenetic reprogramming in dogs. We expect to elucidate the mechanisms of the reprogramming of canine germ cells, leading to a greater understanding of biotechnologic possibilities of these cells. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA, ALINE FERNANDA; PIERI, NAIRA CAROLINE GODOY; MARTINS, DANIELE DOS SANTOS. Step by Step about Germ Cells Development in Canine. ANIMALS, v. 11, n. 3 MAR 2021. Web of Science Citations: 0.

DE SOUZA, ALINE F.; DE RAMOS, ELOISE C.; CURY, FABIO S.; PIERI, NAIRA CAROLINE G.; MARTINS, DANIELE S. The timeline development of female canine germ cells. REPRODUCTION IN DOMESTIC ANIMALS, v. 54, n. 7, p. 964-971, JUL 2019. Web of Science Citations: 0.

GODOY PIERI, NAIRA CAROLINE; FURLANETTO MANCANARES, ANA CAROLINA; DE SOUZA, ALINE FERNANDA; FERNANDES, HUGO; GONELLA DIAZA, ANGELA MARIA; BRESSAN, FABIANA FERNANDES; SANTOS ROBALLO, KELLY CRISTINE; CASALS, JULIANA BARBOSA; BINELLI, MARIO; AMBROSIO, CARLOS EDUARDO; MARTINS, DANIELE DOS SANTOS. Xenotransplantation of canine spermatogonial stem cells (cSSCs) regulated by FSH promotes spermatogenesis in infertile mice. STEM CELL RESEARCH & THERAPY, v. 10, MAY 20 2019. Web of Science Citations: 1.

DE SOUZA, ALINE F.; GODOY PIERI, NAIRA C.; ROBALLO, KELLY C. S.; BRESSAN, FABIANA F.; CASALS, JULIANA B.; AMBROSIO, CARLOS E.; PERECIN, FELIPE; MARTINS, DANIELE S. Dynamics of male canine germ cell development. PLoS One, v. 13, n. 2 FEB 28 2018. Web of Science Citations: 5.