Validation of an in vitro caries induction model and evaluation of a mouthrinse containing Croton doctoris s. Moore essential oil on enamel demineralization and polimcrobyal biofilm composition

• Grant number: 13/12547-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: January 01, 2014
• End date: February 28, 2015
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Fernanda Lourenção Brighenti
• Grantee: Yasmin Etienne Albuquerque
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The aim of this work is to validate an in vitro caries model and use it to evaluate the antimicrobial activity of a mouthrinse containing Croton doctoris essential oil. Studies with polymicrobial biofilm obtained from the saliva of a donor will be performed. Biofilms will be developed on the surface of bovine enamel blocks that will be submitted to a semi-continuous sucrose exposure regimen. For the study validation, the following variables will be analyzed: sucrose concentration in culture medium, maintenance or removal of saliva after initial inoculation, experiment duration, use of neutralizing solution and frequency exposure to the treatment solutions. The variables will be evaluated according to biofilm bacterial concentration (quantification of total anaerobic bacteria, streptococcus of the mutans group, aciduric anaerobic bacteria and lactobacilli), biofilm acidogenicity obtained by culture medium pH assessment and percentage of enamel hardness loss (%SH) obtained by surface microhardness evaluation. The essential oil will be obtained by hydrodistillation and its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) will be determined by broth microdilution method. The developed model will be applied to test the mouthwash containing essential oil at CIM concentration. A mouthwash without essential oil and chlorhexidine digluconate 0.12% (CHX) will be used, respectively, as negative and positive control. The appropriate statistical test for each result will be determined after the completion of the sampling distribution normality test. The significance level will be set at 5%. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALBUQUERQUE, Yasmin Etienne. Validação de um modelo de indução de cárie in vitro e avaliação de um enxaguatório bucal contendo óleo essencial de Croton doctoris S. Moore na desmineralização do esmalte e na composição do biofilme polimicrobiano. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.