The role of airway epithelial cells on experimental models of asthma

• Grant number: 13/23763-0
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: May 01, 2014
• End date: October 31, 2014
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Rodolfo de Paula Vieira
• Grantee: Flavia Regina Greiffo
• Supervisor: Oliver Eickelberg
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
• Institution abroad: Ludwig Maximilian University of Munich (LMU Munich), Germany
• Associated to the scholarship: 12/23305-9 - Effects of aerobic physical training on the chronic allergic pulmonary inflammation in mice: role of purinergic signaling, BP.MS

Abstract

Asthma is a disease characterized by reversible airflow limitation, chronic airway inflammation and remodeling. Airway epithelium is not only a physical barrier in the airways, but actively contributes for the pathogenesis, inflammatory and fibrotic process in asthma. Several inflammatory mediators are involved in the asthmatic response. In this way, purinergic signaling has emerged as an important cellular and molecular via modulating all aspects of asthmatic pathophysiology, through the release of nucleotides leading to activation of purinergic receptors. A growing number of studies have reported the role of purinergic signalling in cells of hematopoietic system (i.e. eosinophils, T-lymphocytes and dendritic cells) in the context of asthma. However, only one study has demonstrated that purinergic signalling (uridine diphosphate via P2Y6 receptor) also modulates asthmatic response through activation of airway epithelium. Therefore, the present study aims to investigate the role of P2Y2 and P2X7 in the airway epithelium activation in the asthmatic response. Then, primary airway epithelial cells from BALB/c mice will be isolated and cultived in an air liquid interface system and will be stimulated with house dust mite (dermatophagoides pteronyssinus) in the context of P2Y2 and P2X7 agonist and antagonist co-stimulation. After cells stimulation, supernatant will be collected for measurment of cytokines and growth factors and the cells will be lysed for protein extraction and evaluation of the expression of P2Y2 and P2X7 receptors through western blotting technique. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALBERCA-CUSTODIO, RICARDO WESLEY; GREIFFO, FLAVIA REGINA; MACKENZIE, BREANNE; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; ANDRADE-SOUSA, ADILSON SANTOS; GRAUDENZ, GUSTAVO SILVEIRA; GOMES SANTOS, ANGELA BATISTA; DAMACENO-RODRIGUES, NILSA REGINA; CASTRO-FARIA-NETO, HUGO CAIRE; ARANTES-COSTA, FERNANDA MAGALHAES; et al. Aerobic Exercise Reduces Asthma Phenotype by Modulation of the Leukotriene Pathway. FRONTIERS IN IMMUNOLOGY, v. 7, JUN 14 2016. (12/15165-2, 14/23196-0, 13/23763-0, 12/23305-9, 14/14604-8, 12/21519-1, 14/09292-7)