Structural elucidation of metabolites from a phthalimide derivative (LAPDESF-SCD03) by high-resolution mass spectrometry (HRMS)

• Grant number: 14/01724-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: May 08, 2014
• End date: December 17, 2014
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Rosangela Gonçalves Peccinini
• Grantee: Michel Leandro de Campos
• Supervisor: Demian R. Ifa
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: York University, Canada
• Associated to the scholarship: 11/23007-5 - Preclinical Pharmacokinetics, pharmacokinetic/pharmacodynamic relationship study and Hepatic and Renal Safety Preliminary Evaluation of Phthalimide Derivative in rats., BP.DR

Abstract

Sickle cell disease is the most common and severe monogenic disorder worldwide. It is characterized by episodes of acute disability and progressive organ damage. In order to treat the symptoms of this disease, a phtalimide derivative, LAPDESF-SCD03, was planned to have nitric oxide donor activity and anti-inflammatory activity, which has been shown in published studies. The characterization of drug metabolites is extremely important since drug metabolite activity is one of the leading causes considered as the reason for unexpected drug safety issues and therefore withdraws in drug research and development. Metabolites can also be the chemical species responsible for the desired biological activity. In this project we propose the use of modern bioanalytical techniques such as high-resolution mass spectrometry (HRMS) for structural elucidation of metabolites from the compound LAPDESF-SCD03 metabolized on rat plasma and hepatocytes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TATA, ALESSANDRA; PEREZ, CONSUELO; CAMPOS, MICHEL L.; BAYFIELD, MARK A.; EBERLIN, MARCOS N.; IFA, DEMIAN R.. Imprint Desorption Electrospray Ionization Mass Spectrometry Imaging for Monitoring Secondary Metabolites Production during Antagonistic Interaction of Fungi. Analytical Chemistry, v. 87, n. 24, p. 12298-12304, DEC 15 2015. (13/15575-9, 14/01724-5)

DE CAMPOS, MICHEL LEANDRO; OLIVEIRA, ISABELA JUNQUEIRA; DAVANCO, MARCELO GOMES; DOS SANTOS, JEAN LEANDRO; PECCININI, ROSANGELA GONCALVES. UHPLC Quantitation Method and In vitro Studies of Two New Phthalimide Derivatives Planned to Treat Sickle Cell Disease. CURRENT PHARMACEUTICAL ANALYSIS, v. 13, n. 4, p. 361-366, 2017. (11/23007-5, 14/01724-5)