Transcriptome analysis of Hevea pauciflora and Hevea benthamiana and Hevea genus orthologs genes identification

• Grant number: 13/20447-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: March 01, 2014
• End date: March 31, 2015
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal Investigator: Anete Pereira de Souza
• Grantee: Guilherme de Toledo e Silva
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Rubber is a strategic raw material for manufacturing of about fifty thousand products, including more than 400 medical devices. Natural rubber characteristics can't be matched by artificial polymers, and some products needs natural rubber in their composition, like medical procedures gloves and airplanes and trucks high-resistance tires. About 2,500 plant species produce latex, where rubbertree (Heveavbrasiliensis (Willd.) Muell. Arg.) is considered natural rubber main source. South American Leaf Blight (SALF), a disease caused by fungi Microcyclos ulei, is currently limiting rubber production in Latin America, also represeting a threat to asian and african H. brasiliensis fields. H. benthamiana and H. pauciflora are considered of extreme importance for H. brasiliensis breeding programs, due to these species natural resistance to M. ulei infection. Until now, no transcriptome study was made focusing on H. benthamiana or H. pauciflora, resulting on few available data on public databases as GenBank. Next-gen sequencing and assembly of H. benthamiana and H. pauciflora transcriptomes, and their subsequent correlation to H. brasiliensis genes throught orthologs groups definition can be a viable strategy for knowledge consolidation on Hevea genus. Moreover, transcriptome study of these two species will supply breeding programs with important genomic resources as microsatellite and single nucleotide polymorphisms molecular markers, allowing their direct employment on H. brasiliensis molecular breeding.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FEIJO ROSA, JOAO RICARDO BACHEGA; MANTELLO, CAMILA CAMPOS; GARCIA, DOMINIQUE; DE SOUZA, LIVIA MOURA; DA SILVA, CARLA CRISTINA; GAZAFFI, RODRIGO; DA SILVA, CICERO CASIMIRO; TOLEDO-SILVA, GUILHERME; CUBRY, PHILIPPE; FRANCO GARCIA, ANTONIO AUGUSTO; et al. QTL detection for growth and latex production in a full-sib rubber tree population cultivated under suboptimal climate conditions. BMC PLANT BIOLOGY, v. 18, OCT 10 2018. (13/20447-0, 12/50491-8)

ZANOTTO VIGNA, BIANCA BACCILI; DE OLIVEIRA, FERNANDA ANCELMO; DE TOLEDO-SILVA, GUILHERME; DA SILVA, CARLA CRISTINA; DO VALLE, CACILDA BORGES; DE SOUZA, ANETE PEREIRA. Leaf transcriptome of two highly divergent genotypes of Urochloa humidicola (Poaceae), a tropical polyploid forage grass adapted to acidic soils and temporary flooding areas. BMC Genomics, v. 17, NOV 11 2016. (13/14903-2, 08/52197-4, 13/20447-0)