Involvement of the central nervous system receptors in muscle hyperalgesia: role of P2X3, AMPA and NMDA receptors

• Grant number: 13/23448-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2014
• End date: December 31, 2014
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Carlos Amilcar Parada
• Grantee: Aline Carolina Salgado Marques
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 11/11064-4 - Development of a new model for the study of muscle hyperalgesia and the involvement of P2X3 and P2X2/3 receptors in muscle hyperalgesia, AP.JP

Abstract

Recently we developed a new model of muscle hyperalgesia induced by sustained isometric contraction of the gastrocnemius muscle of rats. We demonstrate that this response is associated with a hyperalgesic inflammatory process mediated by prostaglandins, sympathomimetic amines and neutrophil migration via activation of endogenous ATP receptor P2X3. While we have shown some of the peripheral mechanisms involved in this response, the central mechanisms have not yet been studied. Considering that the biochemistry and neuroplastic of the Central Nervous System (CNS) are crucial for the transition from acute pain to chronic pain, the aim of this study is to evaluate the involvement of P2X3 receptors, AMPA and NMDA receptors present in the CNS in the hyperalgesic response. To do so, we will test the hypothesis that intrathecal administration of the selective antagonist of P2X3 receptor, A317491, selective antagonist of AMPA receptor, AP5, or selective NMDA receptor antagonist, CNQX, will prevent the mechanical hyperalgesia induced by sustained isometric contraction. Moreover, as our previous studies have shown that P2X3 peripherals are essential for the development of this response, we will analyzed if they are also involved in the maintenance of mechanical hyperalgesia induced by sustained isometric contraction.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AQUINO, BRUNA DE MELO; DA SILVA DOS SANTOS, DIOGO FRANCISCO; JORGE, CAROLINA OCANHA; SALGADO MARQUES, ALINE CAROLINA; TEIXEIRA, JULIANA MAIA; PARADA, CARLOS AMILCAR; GONCALVES OLIVEIRA-FUSARO, MARIA CLAUDIA. P2X3 receptors contribute to muscle pain induced by static contraction by a mechanism dependent on neutrophil migration. PURINERGIC SIGNALLING, v. 15, n. 2, p. 167-175, JUN 2019. (12/10402-6, 13/23448-7, 11/11064-4)