Isolation of cellulose nanofibers from soybean by-products using chemical, enzymatic and physical treatments

• Grant number: 14/02432-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2014
• End date: April 30, 2015
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Milena Martelli Tosi
• Grantee: Marcela da Silva Torricillas
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 09/14610-0 - Use of agro-industrial residues in the elaboration of bioactive films, AP.JP

Abstract

The main aims of the present study are: to obtain and characterize cellulose nanofibers from soybean by-products (straw and bean pods) using different pretreatments (chemical, mechanical and enzymatic). First, the residue will be milled using knife and cryogenic mills. Then, chemical treatment (alkali treatment, bleaching/delignification) of the lignocellulosic biomass to obtain a fraction rich in cellulose. Nanofibers will be obtained by enzymatic hydrolysis ( xylanases and glucanases) and by sonification. These processes will be combined in order to optimize the nanofibers production. The composition of the lignocellulosic material will be determined by High Performance Liquid Chromatography (HPLC ). Two concentrations of enzymes will be tested with different treatment times. The variables studied to optimize this step will be: ( a) determination of reducing sugars and ( b ) zeta potential. The nanofibers cristallinity will be characterized by x-ray diffraction and the morphology observed by transmission electron microscopy (TEM). At the end of the present study, it will be expected to optimize the production of nanofibers with potential application in biodegradable films, aiming the process which have less environmental impact.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTELLI-TOSI, MILENA; TORRICILLAS, MARCELA DA SILVA; MARTINS, MARIA ALICE; GARRIDO DE ASSIS, ODILIO BENEDITO; TAPIA-BLACIDO, DELIA RITA. Using Commercial Enzymes to Produce Cellulose Nanofibers from Soybean Straw. Journal of Nanomaterials, 2016. (16/16222-0, 12/22154-7, 14/02432-8)

MARTELLI-TOSI, MILENA; TORRICILLAS, MARCELA DA SILVA; MARTINS, MARIA ALICE; GARRIDO DE ASSIS, ODILIO BENEDITO; TAPIA-BLACIDO, DELIA RITA. Using Commercial Enzymes to Produce Cellulose Nanofibers from Soybean Straw. Journal of Nanomaterials, v. 2016, p. 10-pg., 2016-01-01. (16/16222-0, 12/22154-7, 14/02432-8)