| Grant number: | 14/03719-9 |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| Start date: | May 01, 2014 |
| End date: | April 08, 2018 |
| Field of knowledge: | Biological Sciences - Immunology |
| Agreement: | Coordination of Improvement of Higher Education Personnel (CAPES) |
| Principal Investigator: | Alexandre Alarcon Steiner |
| Grantee: | Evilin Naname Komegae |
| Host Institution: | Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Associated research grant: | 12/03831-8 - Linking energy balance to systemic inflammation: role of leptin, AP.JP |
| Associated scholarship(s): | 16/15555-6 - Role of leptin in regulation of systemic inflammation, BE.EP.PD |
Abstract There is no doubt that obesity is associated with the risk of systemic inflammation and related syndromes (sepsis). It is possible that leptin, a hormone produced by adipose tissue, is an important link between obesity and inflammation, but the existence of contradictory data and the presence of multiple comorbidities in animals deficient in leptin (or leptin receptor) have hindered the understanding the real role of leptin in systemic inflammation. Our proposal is to clarify the roles ofleptin in systemic inflammation employing strategies of acute silencing and overexpression of the leptin receptor, models in which the roles of leptin may be determined before the onset of comorbidities. Our experimental strategy will evaluate whether leptin at physiological concentrations (not obese) is able to contain the sepsis by exert anti -inflammatory effects by the predominance ofcentral mechanisms, since Wistar rats have the leptin receptor b (RLb) silenced sharply by the use of RNA interference before being induced to endotoxic shock. Evaluate the application of the HPA axis and/or anti -inflammatory vagus nerve , via RLb receptors expressed in the CNS in the containment of systemic inflammation by overexpression of RLb in the CNS of adrenalectomized or subjected to subdiaphragmatic vagotomy rats, respectively . In the contrast, we propose that leptin insupraphysiological concentrations associated with obesity lose predominant action in the CNS by resistance to anti -inflammatory effects of leptin generated, and then pass to contribute to the development of sepsis by the predominance of peripheral mechanisms of action, by action inmacrophages. For this, inbred Lewis rats obese or injected with supraphysiological concentrations of leptin will be depleted of macrophages by use of liposomes. These animals will be repopulate with monocytes that are deficient or overexpressing RLb before induction of endotoxic shock. Therefore, this project by using innovative methodologies designed to test two mechanisms (a central and a peripheral) by which the leptin regulate the systemic inflammation. (AU) | |
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