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Molecular characterization of Monilinia fructicola isolates with different levels of sensitivity to azoxystrobin

Grant number: 14/09472-5
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): July 01, 2014
Effective date (End): September 30, 2014
Field of knowledge:Agronomical Sciences - Agronomy - Plant Health
Principal researcher:Lilian Amorim
Grantee:Isabela Vescove Primiano
Supervisor abroad: Natália A. Peres
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Research place: University of Florida, Gainesville (UF), United States  
Associated to the scholarship:13/20285-0 - Fitness of Monilinia fructicola isolates at different levels of sensitivity to azoxystrobin, BP.MS


The main control of brown rot in peaches (caused by Monilinia fructicola) is by preventive spraying of fungicides, mostly having a single-mode of action. The continuous use of these pesticides implies in drawbacks as the selection of resistance individuals to the active ingredient and, consequently, the efficiency loss of control in the orchards. Population evolution of resistant strains over time can be predicted with competitive ability assays between sensitive and resistant strains. Thus, fitness and stability studies are being developed (FAPESP 2013/20285-0) to check and characterize field isolates and to help in anti-resistance strategies. With the purpose of supplementing and better understanding the results observed in those studies, molecular analysis will be conducted to investigate which mechanism is involved with the resistance of Monilinia fructicola isolates to the strobilurin fungicides and to verify the presence of an intron near by the identified mutation. These analysis will be overseen by professor Dr. Natália Peres, at the Gulf Coast Research and Education Center of the University of Florida, USA. This research group has experience in the area of pathogen resistance to fungicides. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEREIRA, WAGNER V.; PRIMIANO, ISABELA V.; MORALES, RAFAEL G. F.; PERES, NATALIA A.; AMORIM, LILIAN; MAY DE MIO, LOUISE L. Reduced Sensitivity to Azoxystrobin of Monilinia fructicola Isolates From Brazilian Stone Fruits is Not Associated With Previously Described Mutations in the Cytochrome b Gene. PLANT DISEASE, v. 101, n. 5, p. 766-773, MAY 2017. Web of Science Citations: 8.

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