|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||June 01, 2013|
|Effective date (End):||November 30, 2014|
|Field of knowledge:||Agronomical Sciences - Agronomy - Plant Health|
|Principal researcher:||Paulo Cezar Ceresini|
|Grantee:||Samanta Cristiene de Oliveira|
|Home Institution:||Faculdade de Engenharia (FEIS). Universidade Estadual Paulista (UNESP). Campus de Ilha Solteira. Ilha Solteira , SP, Brazil|
This work will seek evidence about the emergence of strobilurin fungicide resistance in populations of the ascomycete fungus Magnaporthe oryzae (anamorphase Pyricularia oryzae), which in the last century, emerged as important pathogen adapted to wheat in southern Brazil and has spread to other countries in South America, such as Bolivia and Paraguay. The management of wheat blast in Brazil, in turn, depends heavily on the application of fungicides due to extremely conditions predisposing to the occurrence of diseases. Between different groups of fungicides used in the management of diseases of wheat, strobilurins has been important. The emergence of resistance to strobilurin fungicides is currently considered common phenomenon in the world, however there is no information about this phenomenon on wheat pathogens in Brazil. We aimed to test the hypothesis that the population of M. oryzae wheat in south-central Brazil remains sensitive to strobilurins or the alternative hypothesis that the use of the fungicide for several years has led the emergence of fungicide resistance in populations associated with the wheat and the weeds in wheat areas in the country. For this, using the marker PCR-RFLP, we will investigate the occurrence of the G143A mutation in the cytochrome b gene which confers resistance to the fungicide QoI strobilurins. We also want to determine if resistance was selected several times as a result of the widespread use of fungicides in wheat. This will be combined with information on the multiloci microsatellite genotypes of strobilurin resistant isolates of M. oryzae to determine if the mutation G143A at cytb is present in more than one genetic background.