Evaluation of host-parasite interaction of the AgMNPV baculovirus with distinct lepidopteran cell lines

• Grant number: 14/03911-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 2014
• End date: August 31, 2016
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Paolo Marinho de Andrade Zanotto
• Grantee: Carla Torres Braconi
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Baculoviridae are arthropod-specific viruses that commonly infect insect orders. During the viral life cycle, two distinct phenotypes are produced: the budded virus (BV) and, the occlusion-derived virus (ODV) for intra and across host spread, respectively. Since the 80's several countries have been using the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) as a biological control agent against the velvet bean caterpillar (A. gemmatalis). In Brazil since the 1980's have been using AgMNPV in the world largest biological control program in soybean plantations. Our previous work revealed that the AgMNPV-2D genome carries at least 152 potential genes and that 149 viral genes are expressed during the infection. Moreover we also showed that a total of 44 proteins are associated to the ODV and 33 to the BV of the AgMNPV-2D. Furthermore, 11 cellular proteins were also identified, which are possibly assorted during viral morphogenesis and carried with the virion. However, when comparing the BV phenotype produced in semi permissive cells, we identified some differences. For example, the late and low level of expression of some genes, and the absence of proteins: AG52, IAP - 2, AG73, P33, 38K, P40, P48 and AG113 in its structure. Therefore, this project aims to understand the mechanisms that influence cell permissiveness to AgMNPV in the cell lineage UFL-AG-286 and SF-9. In addition it aims to understand the differences and dynamics of formation of new BV phenotype in permissive and semi-permissive cell lineage. For this, the specific goals are: (i) identify and quantify all viral and host mRNAs expressed in the early infection in different cell lines of Lepidoptera (ii) test the activity of the anti-apoptotic protein originating from the caterpillar Lonomia oblique to assess changes in permissivity (iii) map the ORFs in silico and compare the differences, (iv) quantify BV proteins based on mass spectrometry using label free methods (v) validate the data and propose pathways and signaling / interaction networks. Permissivity is the main relationship between host-parasite relationships as specificity. Understanding how this process works can help searching for new baculovirus able to be used as biological control, as well as enabling possible future applications such as antigen presentation, alteration of cell tropism, gene delivery and expansion of host range. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE BRITO, ANDERSON FERNANDES; BRACONI, CARLA TORRES; WEIDMANN, MANFRED; DILCHER, MEIK; PEREIRA ALVES, JOAO MARCELO; GRUBER, ARTHUR; DE ANDRADE ZANOTTO, PAOLO MARINHO. The Pangenome of the Anticarsia gernmatalis Multiple Nucleopolyhedrovirus (AgMNPV). GENOME BIOLOGY AND EVOLUTION, v. 8, n. 1, p. 94-108, JAN 2016. (14/03911-7, 11/14537-0, 11/17120-3)

CAIO CÉSAR DE MELO FREIRE; GIUSEPPE PALMISANO; CARLA T BRACONI; FERNANDA R CUGOLA; FABIELE B RUSSO; PATRICIA CB BELTRÃO-BRAGA; ATILA IAMARINO; DANIEL FERREIRA DE LIMA NETO; AMADOU ALPHA SALL; LIVIA ROSA-FERNANDES; et al. NS1 codon usage adaptation to humans in pandemic Zika virus. Memórias do Instituto Oswaldo Cruz, v. 113, n. 5, 2018-05-10. (14/03911-7, 16/03605-9, 14/17766-9, 12/04818-5, 14/06090-4)

OLIVEIRA, DANIELLE B. L.; DURIGON, GIULIANA S.; MENDES, ERICA A.; LADNER, JASON T.; ANDREATA-SANTOS, ROBERT; ARAUJO, DANIELLE B.; BOTOSSO, VIVIANE F.; PAOLA, NICHOLAS D.; NETO, DANIEL F. L.; CUNHA, MARIELTON P.; et al. Persistence and Intra-Host Genetic Evolution of Zika Virus Infection in Symptomatic Adults: A Special View in the Male Reproductive System. Viruses-Basel, v. 10, n. 11, NOV 2018. (13/07467-1, 14/17766-9, 14/27228-4, 14/16333-1, 16/08727-5, 15/25643-7, 14/03911-7, 16/08204-2, 16/20045-7, 13/26942-2, 16/03605-9)