Fluorescence lifetime spectroscopy for in vivo studies

• Grant number: 13/26205-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2014
• End date: January 31, 2016
• Field of knowledge: Physical Sciences and Mathematics - Physics
• Principal Investigator: Cristina Kurachi
• Grantee: Camila de Paula D'Almeida
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 13/07276-1 - CEPOF - Optics and Photonic Research Center, AP.CEPID

Abstract

The analysis of fluorescence lifetime for biological tissues has been presented as a tool of great potential for tissue characterization on diagnostics. Main fluorophores are NADH and FAD, biomolecules involved in cell respiratory chain, which when bing monitored, they present metabolic information. Other molecules will also be investigated, as the photodynamic therapy sensitizers. One of the main advantages of the optical techniques is the in situ interrogation, without the need of tissue removal and processing. The main objective of the present study is to introduce this technique for the evaluation of the tissue response after distinct types of treatment.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAMPOS, CAROLINA DE PAULA; D'ALMEIDA, CAMILA DE PAULA; NOGUEIRA, MARCELO SAITO; MORIYAMA, LILIAN TAN; PRATAVIEIRA, SEBASTIAO; KURACHI, CRISTINA. Fluorescence spectroscopy in the visible range for the assessment of UVB radiation effects in hairless mice skin. Photodiagnosis and Photodynamic Therapy, v. 20, p. 21-27, DEC 2017. (13/07276-1, 13/26205-8)