Inactivation of Borrelia anserina by the action of ultraviolet light associated with riboflavin in blood serum of Gallus gallus domesticus

• Grant number: 14/03303-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2014
• End date: June 30, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Raimundo Souza Lopes
• Grantee: Daniela Marques Maciel Dabus
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Photodynamic therapy as the combination of Ultraviolet Light (UV) and riboflavin is focused for many researches. The avian borreliosis is a disease caused by the spirochete Borrelia anserina (B. anserina), that due to the growth of organic and rustic creations, increases the concern about the emergence of outbreaks of the disease, so the search for alternative therapies becomes of fundamental importance. The objective of the study is to see if whey parasitized with B. anserine following treatment with photodynamic therapy is effective to inactivate or eliminate the pathogen. The study will use a combination of UV with riboflavin. Will be conducted in two stages, the stage in vivo, have a study group, consisting of 37 birds (Gallus gallus domesticus), separated into six groups with six animals in each, which will be formed by light control group (Gcl), which will receive serum parasitized treated with UV and group control vehicle (Gcv) will receive parasitized serum treated with sodium chloride 0.9%, group photosensitizer associated with the vehicle control (Gfs), who will receive parasitized treated with riboflavin serum diluted in sodium chloride 0.9%; health control group (Gcs), which did not receive any treatment or inoculation, disease control group (Gdc) who receive serum parasitized by the strain of B. anserine; treated group (Gt), which receive saline treated infested with UV associated with the riboflavin; step in vitro is using cell culture plates separated the same way as the step in vivo. It is expected that serum infested with B. anserine treated with photodynamic therapy, when inoculated on susceptible animals will not be able to cause disease, and when inoculated into a culture medium not be able to grow. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

DABUS, Daniela Marques Maciel. Inactivation of Borrelia anserine by the action of ultraviolet light associated with riboflavin in blood serum of Gallus gallus domesticus. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.