Consistent liver data and dynamics of protein damage in diabetic rats

• Grant number: 14/14249-3
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: October 15, 2014
• End date: October 14, 2015
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Pedro de Magalhães Padilha
• Grantee: Camila Pereira Braga
• Supervisor: Jiri Adamec
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: University of Nebraska-Lincoln (UNL), United States
• Associated to the scholarship: 11/21672-1 - PROTEOMICS AND METALLOPROTEOMICS TOOLS IN THE CHARACTERIZATION OF PLASMATIC AND HEPATIC BIOMARKERS FROM RATS SUBMITTED TO DIABETES TYPE 1, BP.DR

Abstract

Oxidative stress and protein carbonylation are implicated in various diseases such as diabetes. Therefore, with the accurate identification and quantification of protein carbonylation, together with an understanding of how endogenous antioxidant metalloenzymes are coordinated to the metal ions, we can understand the metabolic response of living systems to biological stimuli, diseases and therapeutic treatments. These approaches may lead to the discovery of new biomarkers. This study aims to analyse the changes in the metalloenzymes involved in oxidative stress of diabetic rats. In order to reach the main objective, in partnership with the University of Nebraska-Lincoln, we aim to develop procedures that work with techniques, such as LC-ESI-MS (liquid chromatography-electrospray ionisation-mass spectrometry) and ICP-MS-(inductively coupled plasma-mass spectrometry), in studies for the identification and characterisation of endogenous antioxidant metalloenzymes. Moreover, we will use a technique that combines 2D-ELFO (Two-dimensional electrophoresis) with hydrazide fluorophores derivatisation to analyse protein carbonylation. The results of this project elucidated some aspects about melloenzymes involved in oxidative stress observed in DM, which are related to metalloproteome studies in diabetic rats. It is, however, expected that the job profile is to provide technical and scientific support in healthcare, enabling the development new strategies for the prevention and treatment of diabetes. (AU)