Smart biointerfaces using sensing and actuating system controlled by Boolean logic

• Grant number: 14/13876-4
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Effective date (Start): November 01, 2014
• Effective date (End): March 31, 2015
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Valber de Albuquerque Pedrosa
• Grantee: Juliane Renata Sempionatto Moreto
• Supervisor: Evgeny Katz
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Research place: Clarkson University, United States
• Associated to the scholarship: 13/12623-2 - Study of stimulus-responsive properties of polymer brush modified with gold nanoparticle, BP.MS

Abstract

The sensing and actuating system has been widely studied aiming environmental and health sensing.This system consists in detecting the target molecule that triggers a drug delivery system. This setting can be used to build systems based on fuel cells, and thus can be implanted in living organisms. These biosensors can be based on Boolean logic. The simulation of logical operators can be performed using enzymatic reactions, for example the "AND" command can be accomplished by complementary reactions, where the enzymatic product of a certain enzyme acts as a substrate to another, so the positive response systems "1" is only possible in the presence of both enzymes, otherwise, the response will be "0". The logical command "OR" can be simulated by parallel enzyme reactions, where the presence of either of the two leads to a "1" response. This project will build a sensing and actuating system controlled by Boolean logic using complementary and parallel enzymatic reactions. ITO electrodes modified with polymer brush will be used as drug delivery. The polymer brushes has the property of changing the configuration of their polymeric chains with external stimuli such as pH change produced by enzymatic reactions; chains may be closed (near the electrode) or extended (away from the electrode), enabling the modulation of drug release. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SEMPIONATTO, JULIANE R.; GAMELLA, MARIA; GUZ, NATALIIA; PINGARRON, JOSE M.; PEDROSA, VALBER A.; MINKO, SERGIY; KATZ, EVGENY. Electrochemically Stimulated DNA Release from a Polymer-Brush Modified Electrode. Electroanalysis, v. 27, n. 9, SI, p. 2171-2179, SEP 2015. (14/13876-4)