In vivo Raman spectroscopy for pathological processes of oral mucosa diagnosis

• Grant number: 14/05978-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2014
• End date: July 31, 2017
• Field of knowledge: Engineering - Biomedical Engineering
• Principal Investigator: Luís Eduardo Silva Soares
• Grantee: Luis Felipe das Chagas e Silva de Carvalho
• Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil
• Associated research grant(s): 17/09766-7 - Raman spectral post-processing for oral tissue discrimination - a step for an automatized diagnostic system, PUB.ART

Abstract

Currently the use of Raman spectroscopy as a technique for optical biopsy is well established. Several studies using the technique as a method for ex vivo analysis and also with cell cultures show a high discriminative power of the technique in comparison between normal and malignant tissues, as well as potentially precancerous lesions and inflammatory processes. Studies ex vivo involving the use of the technique with pathological processes of oral mucosa are among the most discussed, particularly with relevant results. The fact that the oral environment has easily accessible, this technique allows for future in vivo experiments. The aim of this work is the use of Raman spectroscopy for in vivo study and diagnosis of oral disease processes, indeed the spectra obtained in real time and non invasive can provide important information of the biochemical changes that are occurring at that particular pathological process. We also aim to further establish the clinical application of the technique aiding the pathologist in the diagnostic process and optimizing the surgical procedure for professional involved. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARVALHO, LUIS FELIPE C. S.; BONNIER, FRANCK; TELLEZ, CLAUDIO; DOS SANTOS, LAURITA; O'CALLAGHAN, KATE; O'SULLIVAN, JEFF; SOARES, LUIS EDUARDO S.; FLINT, STEPHEN; MARTIN, AIRTON A.; LYNG, FIONA M.; et al. Raman spectroscopic analysis of oral cells in the high wavenumber region. Experimental and Molecular Pathology, v. 103, n. 3, p. 255-262, DEC 2017. (14/05978-1)

CARVALHO, LUIS FELIPE C. S.; NOGUEIRA, MARCELO SAITO; BHATTACHARJEE, TANMOY; NETO, LAZARO P. M.; DAUN, LUCAS; MENDES, THIAGO O.; RAJASEKARAN, RAMU; CHAGAS, MAURILIO; MARTIN, AIRTON A.; SOARES, LUIS EDUARDO S.. In vivo Raman spectroscopic characteristics of different sites of the oral mucosa in healthy volunteers. CLINICAL ORAL INVESTIGATIONS, v. 23, n. 7, p. 3021-3031, JUL 2019. (18/03636-7, 14/05978-1)

MEDEIROS-NETO, L. P.; TELLEZ SOTO, C. A.; CHAGAS, M. J.; CARVALHO, L. F. C.; RAJASEKARAN, R.; MARTIN, A. A.. In vivo Raman spectroscopic characterization of papillary thyroid carcinoma. VIBRATIONAL SPECTROSCOPY, v. 101, p. 1-9, MAR 2019. (14/05978-1)

SILVA GOMES, RAIMUNDO NONATO; BHATTACHARJEE, TANMOY T.; CARVALHO, LUIS FELIPE C. S.; SILVA SOARES, LUIS EDUARDO. Fast monitoring of tooth erosion caused by medicaments used in the treatment of respiratory diseases by ATR-FTIR and mu-EDXRF analysis. Lasers in Medical Science, v. 32, n. 9, p. 2063-2072, DEC 2017. (14/05978-1)

SILVA GOMES, RAIMUNDO NONATO; BHATTACHARJEE, TANMOY T.; CARVALHO, LUIS FELIPE C. S.; SILVA SOARES, LUIS EDUARDO. ATR-FTIR spectroscopy and mu-EDXRF spectrometry monitoring of enamel erosion caused by medicaments used in the treatment of respiratory diseases. MICROSCOPY RESEARCH AND TECHNIQUE, v. 81, n. 2, SI, p. 220-227, FEB 2018. (14/05978-1)

CARVALHO, LUIS FELIPE C. S.; NOGUEIRA, MARCELO SAITO; NETO, LAZARO P. M.; BHATTACHARJEE, TANMOY T.; MARTIN, AIRTON A.. Raman spectral post-processing for oral tissue discrimination - a step for an automatized diagnostic system. BIOMEDICAL OPTICS EXPRESS, v. 8, n. 11, p. 5218-5227, NOV 1 2017. (14/16154-0, 14/05978-1)

MEDEIROS NETO, LAZARO PINTO; DAS CHAGAS E SILVA DE CARVALHO, LUIS FELIPE; DOS SANTOS, LAURITA; TELLEZ SOTO, CLAUDIO ALBERTO; CANEVARI, RENATA DE AZEVEDO; DE OLIVEIRA SANTOS, ANDRE BANDIERA; MELLO, EVANDRO SOBROZA; PEREIRA, MARINA APARECIDA; CERNEA, CLAUDIO ROBERTO; BRANDAO, LENINE GARCIA; et al. Micro-Raman spectroscopic study of thyroid tissues. Photodiagnosis and Photodynamic Therapy, v. 17, p. 164-172, MAR 2017. (14/05978-1)

RYMSZA, TACIANA; RIBEIRO, ELIANE ALINE; DAS CHAGAS E SILVA DE CARVALHO, LUIS FELIPE; BHATTACHARJEE, TANMOY; CANEVARI, RENATA DE AZEVEDO. Human papillomavirus detection using PCR and ATR-FTIR for cervical cancer screening. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v. 196, p. 238-246, MAY 5 2018. (13/14464-9, 09/18440-1, 14/05978-1)

DAS CHAGAS E SILVA DE CARVALHO, LUIS FELIPE; NOGUEIRA, MARCELO SAITO. New insights of Raman spectroscopy for oral clinical applications. ANALYST, v. 143, n. 24, p. 6037-6048, DEC 21 2018. (18/03636-7, 14/05978-1)

CARVALHO, LUIS FELIPE C. S.; DOS SANTOS, LAURITA; BONNIER, FRANCK; O'CALLAGHAN, KATE; O'SULLIVAN, JEFF; FLINT, STEPHEN; NETO, LAZARO P. M.; MARTIN, AIRTON A.; LYNG, FIONA M.; BYRNE, HUGH J.. Can ethanol affect the cell structure? A dynamic molecular and Raman spectroscopy study. Photodiagnosis and Photodynamic Therapy, v. 30, JUN 2020. (14/05978-1)

SILVA GOMES, RAIMUNDO NONATO; BHATTACHARJEE, TANMOY T.; CARVALHO, LUIS FELIPE C. S.; SILVA SOARES, LUIS EDUARDO. ATR-FTIR spectroscopy and mu-EDXRF spectrometry monitoring of enamel erosion caused by medicaments used in the treatment of respiratory diseases. MICROSCOPY RESEARCH AND TECHNIQUE, v. 81, n. 2, p. 8-pg., 2018-02-01. (14/05978-1)